TOXIC AND OSMOTIC EFFECTS OF GLYCEROL ON HUMAN-GRANULOCYTES
TOXIC AND OSMOTIC EFFECTS OF GLYCEROL ON HUMAN-GRANULOCYTES
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DOI：
10.1152/ajpcell.1984.247.5.c382
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发表时间：
1984-01-01
影响因子：
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通讯作者：
MAZUR, P
中科院分区：
文献类型：
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作者：
ARMITAGE, WJ;MAZUR, P
甘油暴露于浓度为0.5M的甘油中会对人的粒细胞造成损伤。为了区分甘油的毒性和有害渗透作用，我们研究了甘油在不同条件下对粒细胞的作用及其稀释作用。甘油在0度时引起的损伤。C仅在孵育(.gtoreq.1h)37度。C.通过降低甘油的添加和稀释率以将计算的细胞体积保持在等渗细胞体积的80%-170%，从而减轻渗透胁迫，这种损伤并未得到改善。但是，当甘油的添加和稀释温度从0度提高到进一步降低渗透胁迫时。到22度。细胞对1M甘油的耐受性有所提高。在摄氏0度时，将接触甘油的时间减少到3分钟或更少。C极大地提高了存活率，但这段时间太短，不能让甘油在细胞内平衡。最后，在培养液中加入额外的不必要的溶质(氯化钠或蔗糖)，将平衡细胞体积减少到等渗细胞体积的60%，使粒细胞在0度的1M甘油中存活30分钟。C，但细胞在37度期间必须保持收缩。C孵育，防止膜完整性的丧失。含有受损粒细胞的悬浮液在37度孵育时形成聚集体。C，而这些聚集体是造成观察到的活力损失的主要部分。
Human granulocytes are damaged by exposure to concentrations of glycerol as low as 0.5 M. The addition of glycerol to granulocytes and its subsequent dilution under various conditions was investigated to try to distinguish between toxic and harmful osmotic effects of glycerol. The lesion caused by glycerol at 0.degree. C was expressed as a loss of plasma membrane integrity (as visualized by fluorescein diacetate) only after incubation (.gtoreq. 1 h) at 37.degree. C. This damage was not ameliorated when osmotic stress was lessened by reducing the rates of addition and dilution of glycerol to keep the computed cell volume within 80-170% of isotonic cell volume. However, when osmotic stress was reduced further by increasing the temperture of addition and dilution of glycerol from 0.degree. to 22.degree. C, the tolerance of the cells to 1 M glycerol increased somewhat. Reducing exposure to glycerol to 3 min or less at 0.degree. C greatly increased survival, but this time was too short to allow glycerol to equilibrate intracellularly. Finally, the presence of extra impermeant solute (NaCl or sucrose) in the medium to reduce the equilibrium cell volume to 60% of isotonic cell volume enabled granulocytes to survive 30-min exposure to 1 M glycerol at 0.degree. C, but cells had to remain shrunken during the 37.degree. C incubation to prevent the loss of membrane integrity. Suspensions that contained damaged granulocytes formed aggregates when incubated at 37.degree. C, and these aggregates were responsible for a major fraction of the observed loss in viability.