Redistribution of 5-lipoxygenase and cytosolic phospholipase A2 to the nuclear fraction upon macrophage activation.
Redistribution of 5-lipoxygenase and cytosolic phospholipase A2 to the nuclear fraction upon macrophage activation.
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巨噬细胞激活后，5-脂氧合酶和胞质磷脂酶 A2 重新分布至核部分。
DOI：
10.1006/bbrc.1993.2227
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发表时间：
1993
影响因子：
3.1
通讯作者：
McNish,RW
中科院分区：
文献类型：
--
作者：
Peters-Golden,M;McNish,RW
细胞溶质磷脂酶A2和5-脂氧合酶从高速上清液中重新分配到颗粒部分，细胞活化后与白三烯合成相关，但这些酶易位的亚细胞位点尚不清楚。在这项研究中，我们打破了休息和离子载体A23187刺激的大鼠腹腔巨噬细胞N2空化和分离裂解物成核，胞浆，和粗膜部分，然后通过免疫印迹分析，这些检查其内容的免疫反应性胞浆磷脂酶A2，5-脂氧合酶，5-脂氧合酶激活蛋白。5-脂氧合酶激活蛋白主要定位于静息和活化细胞的核组分中，而胞浆磷脂酶A2和5-脂氧合酶从静息细胞的胞浆组分重新分布到活化细胞的核组分中。这些数据表明，第一次协调参与内源性花生四烯酸白三烯合成的关键蛋白质的亚细胞定位。
Both the cytosolic phospholipase A2and 5-lipoxygenase enzymes redistribute from the high-speed supernatant to a particulate fraction upon cell activation with associated leukotriene synthesis, but the subcellular site to which these enzymes translocate is not known. In this study, we disrupted resting and ionophore A23187-stimulated rat peritoneal macrophages by N2cavitation and separated lysates into nuclear, cytosolic, and crude membrane fractions; these were then examined by immunoblot analysis for their contents of immunoreactive cytosolic phospholipase A2, 5-lipoxygenase, and 5-lipoxygenase activating protein. 5-Lipoxygenase activating protein was localized predominantly in the nuclear fraction of both resting and activated cells, while both cytosolic phospholipase A2and 5-lipoxygenase redistributed from the cytosol fraction in resting cells to the nuclear fraction in activated cells. These data demonstrate for the first time coordinate subcellular localization of the key proteins involved in leukotriene synthesis from endogenous arachidonate.