Polymorphism of submandibular-sublingual salivary proteins which promote adhesion of Streptococcus mutans serotype-c strains to hydroxyapatite.
Polymorphism of submandibular-sublingual salivary proteins which promote adhesion of Streptococcus mutans serotype-c strains to hydroxyapatite.
复制标题
下颌下舌下唾液蛋白的多态性促进变形链球菌血清型 C 菌株与羟基磷灰石的粘附。
DOI：
10.1177/00220345900690110601
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发表时间：
1990
影响因子：
7.6
通讯作者：
Kent,R
中科院分区：
文献类型：
--
作者：
Kishimoto,E;Hay,DI;Kent,R
此前，我们发现人类下颌舌下(SMSL)唾液含有一种或多种蛋白质，Mr约为300,000道尔顿，它能特异性地促进变形链球菌C型菌株与羟基磷灰石的黏附。此外，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法(SDS-PAGE)显示，黏附促进蛋白(APP)具有异质性。本研究的目的是确定APP是否普遍存在于人类SMSL唾液分泌物中，并表征所指出的异质性。取54例日本受试者经酸刺激后的唾液标本，用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法测定唾液的M值。所有被检查的唾液样本中都存在APP，尽管浓度有显著不同。APP表现为单带(20例)或双带(34例)，平均MR(88条带)为297 kD，范围为248-338 kD。根据MR的应用程序的频率分布图显示了三峰分布，三组的平均MR值、标准差和范围分别为265(S.D.，6.9；范围，248-278)、293(S.D.，6.7；范围280-305)和320(S.D.，7.0；范围，310-338)kD。组内MR的差异可能归因于实验差异，尽管不能排除微观异质性。群体之间的差异可以用三种多态蛋白来解释，即低(L)、中等(I)和高(H)MR值。L、I、H、Li、LH型和IH型共检测到6种表型。Hardy-Weinberg分析表明，表型数据符合单基因三等位基因模型。APP的广泛存在及其调节口腔病原体对牙齿定植的潜力表明，所注意到的浓度和表型的变化可能具有生物学意义。
Previously, we showed that human submandibular-sublingual (SMSL) salivas contain one or more proteins, Mrcirca 300,000 daltons, which specifically promote adhesion of Streptococcus mutans serotype-c strains to hydroxyapatite. Also, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that the adhesion-promoting proteins (APPs) exhibit heterogeneity. The aims of the present study were to determine whether APPs are generally present in human SMSL salivary secretions and to characterize the noted heterogeneity. Acid-stimulated SMSL saliva samples were obtained from 54 Japanese subjects, and M, values were obtained by SDS-PAGE. APPs were present in all saliva samples examined, though at significantly different concentrations. The APPs occurred as either single (20 subjects) or double bands (34 subjects), with a mean Mr(88 bands) of 297 kD and a range of 248-338 kD. A plot of the frequency distribution of the APPs according to Mr showed a trimodal distribution, with mean Mrvalues, standard deviations, and ranges for the three groups being 265 (S.D., 6.9; range, 248-278), 293 (S.D., 6.7; range, 280-305), and 320 (S.D., 7.0; range, 310-338) kD. Variations of Mr within groups may be attributed to experimental variation, although microheterogeneity cannot be excluded. Differences between groups can best be explained in terms of three polymorphic proteins, with low (L), intermediate (I), and high (H) Mr values. Six phenotypes were detected with L, I, H, LI, LH, and IH Mrbands. A Hardy-Weinberg analysis showed that the phenotype data fit a single-gene, three-alleles model. The widespread occurrence of the APPs and their potential to modulate colonization of teeth by an oral pathogen suggest that the noted variations in concentrations and phenotypes may be of biological significance.