c-Fos induction in response to taste stimuli previously paired with amphetamine or LiCl during taste aversion learning.
c-Fos induction in response to taste stimuli previously paired with amphetamine or LiCl during taste aversion learning.
复制标题
在味觉厌恶学习过程中，c-Fos 诱导响应先前与安非他明或 LiCl 配对的味觉刺激。
DOI：
10.1016/0006-8993(94)01421-d
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Bernstein,IL
中科院分区：
文献类型：
--
作者：
Swank,MW;Schafe,GE;Bernstein,IL
安非他明和氯化锂（LiCl）都是大鼠条件味觉厌恶（CTA）建立的有效非条件刺激（USs）。然而，这些药物的作用机制有很大的不同，对LiCl的反应至关重要的是后期区域和相关的呕吐回路，而不是安非他明。c-Fos免疫组织化学用于确定在药物给药期间和在使用安非他明或LiCl作为美国药物的CTA表达期间激活的脑区域。LiCl在孤立束（NTS）核中诱导密集的c- fos样免疫反应性（c-FLI），而安非他明在该区域仅诱导光染色。然而，与安非他明配对的条件刺激（CS）糖精溶液与NTS中的c-FLI相关，其模式与lcl配对的CS非常相似。这表明，c-Fos在条件反射后对味觉CS的表达模式通常具有厌恶条件反射的特征，并且似乎不代表对药物特定非条件作用的条件反应的匹配。为了进一步研究这种条件反应，将c-FLI对厌恶糖精CS的反应与对天生厌恶的盐酸奎宁的反应进行了比较。虽然动物的行为摄取反应非常相似，但糖精CS诱导NTS中c-FLI显著升高，而奎宁则没有。因此，由于条件作用而变得令人厌恶的味道诱导了NTS中的c-FLI表达，而固有厌恶的味道则没有。
Amphetamine and lithium chloride (LiCl) are both effective unconditioned stimuli (USs) in the establishment of conditioned taste aversions (CTA) in the rat. However, the mechanism of action of these drugs is quite different with the area postrema and related emetic circuitry critical to the response to LiCl but not amphetamine. c-Fos immunohistochemistry was used to define brain regions activated during drug administration and during expression of a CTA using either amphetamine or LiCl as the US drug. Administration of LiCl induced dense c-Fos-like immunoreactivity (c-FLI) in the nucleus of the solitary tract (NTS) while amphetamine induced only light staining in this area. A conditioned stimulus (CS) saccharin solution paired with amphetamine, however, was associated with c-FLI in NTS in a pattern quite similar to that seen to a LiCl-paired CS. This suggests that the pattern of c-Fos expression to a taste CS after conditioning is characteristic of aversion conditioning, in general, and appears not to represent a matching of the conditioned response to specific unconditioned effects of the drug. To examine this conditioned response further, c-FLI to the aversive saccharin CS was compared to the response to quinine hydrochloride, which is innately aversive. Although behaviorally the animals' ingestive responses were quite similar, the saccharin CS induced significant elevations of c-FLI in NTS whereas the quinine did not. Thus, a taste which had become aversive by virtue of conditioning induced c-FLI expression in NTS while a taste which was inherently aversive did not.