Expression of growth/differentiation factor 11, a new member of the BMP/TGFβ superfamily during mouse embryogenesis
Expression of growth/differentiation factor 11, a new member of the BMP/TGFβ superfamily during mouse embryogenesis
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DOI：
10.1016/s0925-4773(98)00205-6
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发表时间：
1999-02-01
影响因子：
2.6
通讯作者：
Joyner, A
中科院分区：
文献类型：
--
作者：
Nakashima, M;Toyono, T;Joyner, A
我们已经克隆和表征的骨形态发生蛋白/转化生长因子β（BMP/TGF β）超家族，生长分化因子11（Gdf 11），从大鼠切牙牙髓RNA逆转录聚合酶链反应，使用简并引物的新成员。Gdf 11编码的成熟羧基末端结构域与Gdf 8最密切相关，与小鼠基因有90%相同。北方印迹分析显示Gdf 11在成人牙髓和脑中表达。切片和整装胚胎的原位杂交表明，Gdf 11首先在性交后8.5天（DPC）的限制性结构域中强烈表达，此时它在尾芽中的表达最高。在10.5dpc时，它在鳃弓、肢芽、尾芽和后背神经管中表达。后来，它在终末分化的成牙本质细胞、鼻上皮、视网膜和大脑的特定区域中表达。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
We have cloned and characterized a new member of the bone morphogenetic protein/transforming growth factor beta (BMP/TGF beta) superfamily, growth differentiation factor 11 (Gdf11), from rat incisor pulp RNA by reverse transcription-polymerase chain reaction using degenerate primers. The mature carboxyl-terminal domain encoded by Gdf11 is most closely related to Gdf8, being 90% identical to the mouse gene. Northern blot analysis revealed Gdf11 is expressed in adult dental pulp and brain. In situ hybridization of sections and whole-mount embryos demonstrated Gdf11 is first strongly expressed in restricted domains at 8.5 days post coitus (dpc) when it is highest in the tail bud. At 10.5 dpc, it is expressed in the branchial arches, limb bud, tail bud and posterior dorsal neural tube. Later, it is expressed in terminally-differentiated odontoblasts, the nasal epithelium, retina and specific regions of the brain. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.