Exosome-mediated diagnosis of pancreatic cancer using lectin-conjugated nanoparticles bound to selective glycans
Exosome-mediated diagnosis of pancreatic cancer using lectin-conjugated nanoparticles bound to selective glycans
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DOI：
10.1016/j.bios.2021.112980
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发表时间：
2021-01-12
影响因子：
12.6
通讯作者：
Choi, Jonghoon
中科院分区：
文献类型：
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作者：
Choi, Yonghyun;Park, Uiseon;Choi, Jonghoon
转移性癌细胞中上调的糖基化的独特特征可以形成开发用于靶向和诊断特定癌症的新生物标志物的基础。本研究介绍了一种胰腺癌细胞来源的外泌体检测技术，该技术基于凝集素与外泌体表面独特聚糖谱的特异性结合。将对唾液酸或岩藻糖具有高特异性亲和力的凝集素连接到双功能Janus纳米颗粒（JNPs）上，这有助于在微流体装置中与胰腺癌细胞来源的外泌体相互作用。在这里，我们表明，来自两个细胞系的胰腺癌细胞来源的外泌体和从诊断为胰腺癌的患者收集的血浆样品被成功地捕获在凝集素缀合的JNPs上，其亲和力与CA 19 -9（一种常规抗体）的亲和力相当。此外，使用我们的平台进行外泌体检测可以区分转移性和非转移性胰腺癌细胞。这项研究开启了基于胰腺癌细胞来源的外泌体的聚糖特性实现新的早期诊断标志物的可能性。
The unique profile of upregulated glycosylation in metastatic cancer cells may form the basis for the development of new biomarkers for the targeting and diagnosis of specific cancers. This study introduces a pancreatic cancer cell-derived exosome detection technology, which is based on the specific binding of lectins to distinctive glycan profiles on the surface of exosomes. Lectins with a high and specific affinity for sialic acid or fucose were attached to bifunctional Janus nanoparticles (JNPs), which facilitated interactions with pancreatic cancer cell-derived exosomes in a microfluidic device. Here, we show that pancreatic cancer cell-derived exosomes from two cell lines and plasma samples collected from patients diagnosed with pancreatic cancer were successfully captured on the lectin-conjugated JNPs with affinities that were comparable to those of CA19-9, a conventional antibody. In addition, exosome detection using our platform could differentiate between metastatic and nonmetastatic pancreatic cancer cells. This study opens the possibility to achieve a new early diagnosis marker based on the glycan properties of pancreatic cancer cell-derived exosomes.