First isolation of human adenovirus type 85 by molecular analysis of adenoviruses in cases of urethritis
First isolation of human adenovirus type 85 by molecular analysis of adenoviruses in cases of urethritis
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DOI：
10.1099/jmm.0.001149
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发表时间：
2020-01-01
影响因子：
3
通讯作者：
Furubayashi, Keiichi
中科院分区：
文献类型：
--
作者：
Hiroi, Satoshi;Kawahata, Takuya;Furubayashi, Keiichi
导论.人腺病毒（humanadenovirus，HAdV）是性传播性尿道炎的一个潜在病因。我们的目的是通过分子特征研究日本大坂地区与尿道炎相关的HAdV。尿液样本来自2015年至2018年的男性尿道炎患者。对分离菌株进行分子生物学分析，并对临床标本进行实时聚合酶链反应分析。将分离株分为5个类型，分别属于D种（18例）和E种（1例）。HAdV-85（D种）首次在尿道炎病例中检出。随访结果显示，4例患者在首次采样后半个月内从尿液中分离到HAdV，2例患者在1个月后可检测到病毒DNA。从尿道炎病例中检测到的HAdV类型与呼吸道和眼部HAdV感染有关，而被鉴定为结膜炎原因的新型HAdV类型也会引起尿道炎。HAdV生殖器感染后1个月内应避免性接触。
Introduction. Human adenovirus (HAdV) has been reported as a potential cause of sexually transmitted urethritis.Aim. We aimed to investigate HAdVs associated with urethritis in Osaka, Japan through molecular characterization.Methodology. Urine samples were obtained from male patients with urethritis from 2015 to 2018. Molecular analysis of the isolated strains and follow-up real-time polymerase chain reaction analyses of the clinical samples were performed.Results. The isolates were classified into five types belonging to species D (18 cases) or E (one case). HAdV-85 (species D) was detected for the first time in a urethritis case. Follow-up examination demonstrated that HAdV was isolated from urine samples half a month after the first sampling in four cases, and that viral DNA could be detected after 1 month in two cases.Conclusion. The HAdV types detected from urethritis cases were related to respiratory and ocular HAdV infections, while a novel HAdV type identified as a cause of conjunctivitis also causes urethritis. Sexual contact should be avoided for 1 month after HAdV genital infection.