Molecular Investigation for Bacterial and Protozoan Tick-Borne Pathogens in Wild Boars (Sus scrofa) from Southern Germany
Molecular Investigation for Bacterial and Protozoan Tick-Borne Pathogens in Wild Boars (Sus scrofa) from Southern Germany
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DOI：
10.1089/vbz.2013.1495
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发表时间：
2014-05-01
影响因子：
2.1
通讯作者：
Overzier, Evelyn
中科院分区：
文献类型：
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作者：
Silaghi, Cornelia;Pfister, Kurt;Overzier, Evelyn
野猪（Sus scrofa）被认为参与了蜱传病原体嗜吞噬细胞无形体（Anaplasma phagocytophilum）的地方流行周期。这一观察结果提出了一个问题，即它们是否是A.嗜吞噬细胞菌和潜在的其他蜱传病原体的公共卫生相关性。本研究的目的是调查来自德国南部森林的野猪和它们身上的蜱是否存在A。嗜吞噬细胞菌，嗜噬细胞菌属，米库兰新埃立克体，立克次体属，广义伯氏疏螺旋体（s.l.），疏螺旋体属回归热组和巴氏杆菌属。为此，对2010年10月至2013年2月采集的24头野猪进行了分子生物学研究。A的DNA。24只野猪中有3只（12.5%）和16只蜱中有4只（25%）检出嗜吞噬细胞菌。在任何野猪身上都没有发现其他病原体的DNA，但是立克次体，B。burgdorferi s.l.，和坎德。N. mikurensis在每一个被调查的蜱中被发现。本研究测定了A.来自一个脾脏和两个蜱的嗜吞噬细胞原虫与来自人无形体病病例的GenBank条目显示100%的相似性（登录号：U02521和AY886761）。第三只蜱虫的序列与之前从同一研究区域的篦麻硬蜱和鹿中获得的序列100%相似。检测到一种潜在的人类致病性A.野猪中的嗜吞噬细胞菌变体证实了先前的发现，并具有公共卫生意义。据我们所知，这是A.在德国野猪中发现嗜吞噬细胞菌。野猪是否支持A.在未来的系统研究中，需要进一步关注与人类疾病有关的嗜吞噬细胞菌变体。
Wild boars (Sus scrofa) have been suggested to be involved in the enzootic cycle of the tick-borne pathogen Anaplasma phagocytophilum. This observation raises the question whether they serve as reservoir hosts for A. phagocytophilum and potentially for other tick-borne pathogens of public health relevance. The aim of this study was to investigate wild boars and their ticks from a forest site in southern Germany for the presence of A. phagocytophilum, Candidatus Neoehrlichia mikurensis, Rickettsia spp., Borrelia burgdorferi sensu lato (s.l.), Borrelia spp. of the relapsing fever group, and Babesia spp. Therefore, 24 wild boars collected from October, 2010, to February, 2013, were investigated by molecular methods. DNA of A. phagocytophilum was detected in three out of 24 (12.5%) wild boars and in four out of 16 (25%) ticks. DNA of none of the other pathogens was found in any wild boar, but Rickettsia spp., B. burgdorferi s.l., and Cand. N. mikurensis were found in one of the investigated ticks each. Sequences of the partial 16S rRNA gene of A. phagocytophilum from one spleen and two ticks showed 100% similarity to GenBank entries from human anaplasmosis cases (accession nos. U02521 and AY886761). The sequence from the third tick was 100% similar to sequences obtained from Ixodes ricinus and roe deer from the same study area previously. Detecting a potentially human pathogenic A. phagocytophilum variant in wild boar confirms previous findings and is of public health interest. To our knowledge, this is the first report of A. phagocytophilum in wild boars in Germany. Whether wild boars support the enzootic cycle of A. phagocytophilum variants involved in human disease requires further attention in future systematic studies.