A dual-color far-red to near-infrared firefly luciferin analogue designed for multiparametric bioluminescence imaging.
A dual-color far-red to near-infrared firefly luciferin analogue designed for multiparametric bioluminescence imaging.
复制标题
双色远红色至近红外萤火虫荧光素类似物，专为多参数生物发光成像而设计。
DOI：
10.1002/anie.201405955
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发表时间：
2014-11-24
期刊：
影响因子：
--
通讯作者：
Pule MA
中科院分区：
文献类型：
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作者：
Jathoul AP;Grounds H;Anderson JC;Pule MA
红移生物发光发射器可以改善 活体组织的穿透性和信号量化，并导致甲虫荧光素类似物的开发，这种类似物可以用萤火虫荧光素酶(Fluc)诱导红移生物发光。然而，与天然荧光素不同的是，没有一种荧光素能与不同的荧光素酶发出不同的颜色。我们合成并测试了甲虫荧光素的第一个双色、远红外线和近红外(NIR)发射类似物，它类似于天然荧光素，具有pH 相关的荧光光谱，并用不同的工程荧光素酶发出不同颜色的生物发光。我们的类似物用不同的λ突变体产生了不同的远红到近红外发射最大值，最高可达706 nm。这种发射是在没有使用共振能量转移受体的情况下报道的最红移的生物发光。这一改进将允许使用多参数深层组织生物发光成像更有效地探测组织。
Red-shifted bioluminescent emitters allow improved in vivo tissue penetration and signal quantification, and have led to the development of beetle luciferin analogues that elicit red-shifted bioluminescence with firefly luciferase (Fluc). However, unlike natural luciferin, none have been shown to emit different colors with different luciferases. We have synthesized and tested the first dual-color, far-red to near-infrared (nIR) emitting analogue of beetle luciferin, which, akin to natural luciferin, exhibits pH dependent fluorescence spectra and emits bioluminescence of different colors with different engineered Fluc enzymes. Our analogue produces different far-red to nIR emission maxima up to λmax=706 nm with different Fluc mutants. This emission is the most red-shifted bioluminescence reported without using a resonance energy transfer acceptor. This improvement should allow tissues to be more effectively probed using multiparametric deep-tissue bioluminescence imaging.