Voltage-sensitive dye imaging during functional development of the embryonic nervous system: a brief review with special thanks to Professor Larry Cohen.
Voltage-sensitive dye imaging during functional development of the embryonic nervous system: a brief review with special thanks to Professor Larry Cohen.
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胚胎神经系统功能发育过程中的电压敏感染料成像：简要回顾，特别感谢拉里·科恩教授。
DOI：
10.1117/1.nph.2.2.021009
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发表时间：
2015
期刊：
影响因子：
5.3
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Momose-Sato;Y. and Sato;K.
研究胚胎神经系统的发育组织是神经科学领域的主要挑战之一。尽管它们具有重要意义，但脊椎动物胚胎中枢神经系统(CNS)的功能研究一直受到与传统电生理方法相关的技术限制的阻碍。科恩博士和他的同事开发的使用电压敏感染料的光学技术的出现，使活细胞的电活动能够得到非侵入性的监测，并有助于同时记录多个区域的神经反应。利用光学记录技术，现在可以跟踪胚胎中枢神经系统的功能组织，并成像参与这个神经网络形成的时空动力学。本文对胚胎中枢神经系统的光学研究进行了简要综述，重点介绍了神经回路形成的方法学研究，说明了快速电压敏感染料成像是阐明胚胎中枢神经系统功能组织的有力工具。
Investigating the developmental organization of the embryonic nervous system is one of the major challenges in the field of neuroscience. Despite their significance, functional studies on the vertebrate embryonic central nervous system (CNS) have been hampered by the technical limitations associated with conventional electrophysiological methods. The advent of optical techniques using voltage-sensitive dyes, which were developed by Dr. Cohen and his colleagues, has enabled electrical activity in living cells to be monitored noninvasively and also facilitated the simultaneous recording of neural responses from multiple regions. Using optical recording techniques, it is now possible to follow the functional organization of the embryonic CNS and image the spatiotemporal dynamics involved in the formation of this neural network. We herein briefly reviewed optical studies on the embryonic CNS with a special emphasis on methodological considerations and the study of neuronal circuit formation, which demonstrates the utility of fast voltage-sensitive dye imaging as a powerful tool for elucidating the functional organization of the embryonic CNS.