Biomolecular Interactions and Biological Responses of Emerging Two-Dimensional Materials and Aromatic Amino Acid Complexes.
Biomolecular Interactions and Biological Responses of Emerging Two-Dimensional Materials and Aromatic Amino Acid Complexes.
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DOI：
10.1021/acsami.6b04571
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发表时间：
2016-06
影响因子：
9.5
通讯作者：
Sai Sunil Kumar Mallineni;J. Shannahan;Achyut J. Raghavendra;A. Rao;Jared M Brown;R. Podila
中科院分区：
文献类型：
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作者：
Sai Sunil Kumar Mallineni;J. Shannahan;Achyut J. Raghavendra;A. Rao;Jared M Brown;R. Podila
本研究通过实验研究了芳香氨基酸，即酪氨酸、色氨酸和苯丙氨酸与新型二维（2D）材料，包括石墨烯、氧化石墨烯（GO）和氮化硼（BN）的相互作用。利用光致发光、微拉曼光谱和循环伏安法研究了二维材料与氨基酸之间相互作用的性质和可能的电荷转移。石墨烯和氧化石墨烯通过π-π堆叠、电荷转移和氢键与芳香氨基酸发生了强烈的相互作用。特别是，我们观察到氧化石墨烯/石墨烯与苯丙氨酸和色氨酸的相互作用中，物理和化学吸附都很突出，而酪氨酸在石墨烯和氧化石墨烯上表现出很强的化学吸附。相比之下，BN晶格中N原子周围的π电子云局域化，使得BN几乎没有相互作用。最后，氨基酸在二维材料上的吸附被观察到在活性氧生成方面显著改变了它们的生物反应。更重要的是，这些生物反应的变化遵循了物理和化学吸收测量中观察到的相同趋势。
The present work experimentally investigates the interaction of aromatic amino acids viz., tyrosine, tryptophan, and phenylalnine with novel two-dimensional (2D) materials including graphene, graphene oxide (GO), and boron nitride (BN). Photoluminescence, micro-Raman spectroscopy, and cyclic voltammetry were employed to investigate the nature of interactions and possible charge transfer between 2D materials and amino acids. Graphene and GO were found to interact strongly with aromatic amino acids through π-π stacking, charge transfer, and H-bonding. Particularly, it was observed that both physi and chemisorption are prominent in the interactions of GO/graphene with phenylalanine and tryptophan while tyrosine exhibited strong chemisorption on graphene and GO. In contrast, BN exhibited little or no interactions, which could be attributed to localized π-electron clouds around N atoms in BN lattice. Lastly, the adsorption of amino acids on 2D materials was observed to considerably change their biological response in terms of reactive oxygen species generation. More importantly, these changes in the biological response followed the same trends observed in the physi and chemisorption measurements.