METHOD FOR PREPARING ISOLATED GLANDS FROM RABBIT GASTRIC-MUCOSA
METHOD FOR PREPARING ISOLATED GLANDS FROM RABBIT GASTRIC-MUCOSA
复制标题
DOI：
10.1111/j.1748-1716.1976.tb10184.x
复制
发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
OBRINK, KJ
中科院分区：
文献类型：
--
作者：
BERGLINDH, T;OBRINK, KJ
提出了一种从兔胃粘膜体中分离胃腺的方法。麻醉兔胃经主动脉高压灌注生理盐水，取出并排空。剥离粘膜，切成小块，转移到1 mg/ml的胶原酶溶液中。37℃下90 min后，大量离体胃腺和细胞自由分离。通过简单的清洗程序，腺体从细胞污染和胶原酶中解放出来。通过染料排除技术、耗氧量和电解质含量证明胃腺是有活力的。为了鉴定腺细胞，使用了常用的染色技术和电子显微镜。鉴定出四种细胞类型，即壁细胞、酶原细胞、黏液颈细胞和部分内分泌细胞。腺体细胞的胞内形态与完整胃粘膜无明显差异。正如耗氧量增加（ED - 50 = 3 × 10 - 6M）所揭示的那样，组胺可以以剂量反应的方式刺激腺体。这种分离的胃腺制剂可能为胃生理学的新方法提供有用的工具。
A method for isolating gastric glands from the corpus of the rabbit gastric mucosa is presented. The stomach of an anesthetized rabbit was perfused with saline under high pressure through the aorta, taken out and emptied. The mucosa was stripped off, minced into small pieces and transferred to a 1 mg/ml collagenase solution. After 90 min at 37°C, a large number of isolated gastric glands and cells were separated free. By a simple washing procedure the glands were freed from cell contamination and collagenase. The gastric glands were viable, as demonstrated by dye exclusion technique, oxygen consumption and electrolyte content. For identification of the glandular cells both common staining techniques and electron microscopy were used. Four types of cells were identified, viz. parietal cells, zymogen cells, mucous neck cells and some endocrine cells. The intracellular morphology of the glandular cells did not differ significantly from that seen in intact gastric mucosa. The glands could be stimulated with histamine, in a dose‐response manner, as revealed by the increase in oxygen consumption (ED‐50 equal 3 × 10‐6M). This isolated gastric gland preparation may serve as a useful tool for new approaches in gastric physiology.