The cotton WRKY transcription factor (GhWRKY33) reduces transgenic Arabidopsis resistance to drought stress
The cotton WRKY transcription factor (GhWRKY33) reduces transgenic Arabidopsis resistance to drought stress
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DOI：
10.1038/s41598-018-37035-2
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发表时间：
2019-01-24
影响因子：
4.6
通讯作者：
Li, Xue-Bao
中科院分区：
文献类型：
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作者：
Wang, Na-Na;Xu, Shang-Wei;Li, Xue-Bao
棉花作为纺织工业中天然纤维的重要来源，其纤维品质和产量往往受到干旱条件的制约，因为世界上大多数棉花植物生长在缺水地区。WRKY转录因子调节植物的多种生理过程，包括干旱胁迫反应。然而，对WRKY基因在棉花中如何响应干旱胁迫知之甚少。我们前期的研究表明GhWRKY33是叶片特异性的，受干旱胁迫诱导。在这项研究中，我们的数据表明，GhWRKY33蛋白定位于细胞核，并能够结合到“W盒”的目标启动子的顺式作用元件。在干旱胁迫下，GhWRKY33过表达的转基因拟南芥由于失水速度快，比野生型枯萎得更快。此外，GhWRKY33转基因植株表现出更强的阿坝耐受性，相对于野生型。干旱或阿坝处理改变了转GhWRKY33基因拟南芥中一些干旱胁迫相关基因和ABA响应基因的表达。综上所述，我们的研究结果表明GhWRKY33可能作为负调节因子介导植物对干旱胁迫的响应，并参与阿坝信号通路。
As the important source of natural fibers in the textile industry, cotton fiber quality and yield are often restricted to drought conditions because most of cotton plants in the world grow in the regions with water shortage. WRKY transcription factors regulate multiple plant physiological processes, including drought stress response. However, little is known of how the WRKY genes respond to drought stress in cotton. Our previous study revealed GhWRKY33 is leaf-specific and induced by drought stress. In this study, our data showed GhWRKY33 protein localizes to the cell nucleus and is able to bind to "W-box" cis-acting elements of the target promoters. Under drought stress, GhWRKY33 overexpressing transgenic Arabidopsis was withered much more quickly than wild type due to faster water loss. Moreover, GhWRKY33 transgenic plants displayed more tolerance to abscisic acid (ABA), relative to wild type. Expression of some drought stress-related genes and ABA-responsive genes were changed in the GhWRKY33 transgenic Arabidopsis with drought or ABA treatment. Collectively, our findings indicate that GhWRKY33 may act as a negative regulator to mediate plant response to drought stress and to participate in the ABA signaling pathway.