Expression of intermediate filament proteins in subtypes of renal cell carcinomas and in renal oncocytomas. Distinction of two classes of renal cell tumors.
Expression of intermediate filament proteins in subtypes of renal cell carcinomas and in renal oncocytomas. Distinction of two classes of renal cell tumors.
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中间丝蛋白在肾细胞癌亚型和肾嗜酸细胞瘤中的表达。
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
W. Thoenes
中科院分区：
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作者：
S. Pitz;R. Moll;S. Störkel;W. Thoenes
我们研究了不同的细胞角蛋白（CK）多肽的表达，以及波形蛋白在人类肾细胞癌的各种亚型和肾嗜酸细胞瘤，应用二维凝胶电泳和免疫细胞化学，通过使用多肽特异性单克隆抗体。根据世界卫生组织的指南对肿瘤进行分类，主要根据最近提出的细胞形态学标准进行了一些修改。所有透明细胞癌（G I，G II; N = 20）共表达CKs nos。8和18，波形蛋白，CK编号19存在于20例中的13例中，并表现出不均匀的分布。去分化癌（G III; N = 8）也共表达CKs nos。8和18以及波形蛋白，但除此之外，还显示CK编号19，在许多情况下，CK编号7;在1例病例中，仅表达波形蛋白。嗜酸性粒细胞癌（N = 3）和嗜碱性粒细胞癌（小细胞立方; N = 6）均含有CKs nos。8和18，波形蛋白的共表达是这些肿瘤的一致特征;在所有这些病例中发现CK编号19，而CK编号7在大多数病例中存在。在嫌色细胞癌（N = 8）中，与所有其他癌类型相反，在肿瘤细胞中未检测到波形蛋白，仅CKs nos. 8，18，以及可变程度7，存在。同样，嗜酸细胞瘤（N = 8）缺乏波形蛋白，仅显示CKs nos。8和18。在最后两种肿瘤类型中发现明显的散在CK 19阳性细胞。除单纯上皮型CK外，无CK多肽（nos. 7、8、18和19）在所研究的任何肿瘤中检测到。这些结果表明，在肾细胞肿瘤中，中间丝蛋白的表达与特定的形态学表现显著相关。CKs nos. 8和18，波形蛋白是肾细胞癌最常见亚型的令人惊讶的一致特征，CK no. 19在个体肿瘤内和不同肿瘤之间表现出显著的表达异质性，该CK的表达模式与肿瘤亚型相关。嫌色细胞癌和嗜酸细胞瘤中波形蛋白的持续缺失使得将其定义为单独的一类肾细胞肿瘤成为可能。这一发现支持了嫌色细胞癌代表一种独特的肿瘤实体的观点，并指出其与良性嗜酸细胞瘤以及正常肾小管的密切表型关系。
We examined the expression of the diverse cytokeratin (CK) polypeptides as well as vimentin in human renal cell carcinomas of various subtypes and in renal oncocytomas by applying both two-dimensional gel electrophoresis and immunocytochemistry by using polypeptide-specific monoclonal antibodies. The tumors were classified according to the guidelines of the World Health Organization, with some modifications based primarily on recently proposed cytomorphological criteria. All clear cell carcinomas (G I, G II; N = 20) co-expressed CKs nos. 8 and 18, and vimentin, with CK no. 19 being present in 13 of the 20 cases and exhibiting a heterogeneous distribution. Dedifferentiated carcinomas (G III; N = 8) also co-expressed CKs nos. 8 and 18 as well as vimentin, but in addition, exhibited CK no. 19 and, in many cases, CK no. 7; in 1 case, only vimentin was expressed. Both eosinophilic-granular (N = 3) and basophilic (small cell cuboidal; N = 6) carcinomas contained CKs nos. 8 and 18, and the co-expression of vimentin was a consistent feature of these tumors; CK no. 19 was found in all of these cases, while CK no. 7 was present in the majority. In chromophobe cell carcinomas (N = 8), in contrast to all of the other carcinoma types, no vimentin was detected in the tumor cells, with only CKs nos. 8, 18, and to a variable extent 7, being present. Similarly, oncocytomas (N = 8) lacked vimentin and exhibited only CKs nos. 8 and 18. Conspicuous scattered CK no. 19-positive cells were found in these two last tumor types. No CK polypeptides other than simple-epithelium-type CKs (nos. 7, 8, 18, and 19) were detected in any of the tumors studied. These results indicate that, in renal cell tumors, the expression of intermediate-filament proteins is strikingly correlated with the specific morphologic appearance. While the co-expression of CKs nos. 8 and 18 and vimentin was a surprisingly consistent feature of the most common subtypes of renal cell carcinomas, CK no. 19 exhibited remarkable heterogeneity of expression both within individual tumors and between different tumors, the expression patterns of this CK being correlated to the tumor subtypes. The consistent absence of vimentin in chromophobe cell carcinomas and oncocytomas makes it possible to define these as a separate class of renal cell tumors. This finding supports the view that chromophobe cell carcinomas represent a distinct tumor entity and points to their close phenotypic relationship to benign oncocytomas as well as to normal renal tubules.