CHARACTERIZATION OF AN ISOGENIC SET OF METHICILLIN-RESISTANT AND SUSCEPTIBLE MUTANTS OF STAPHYLOCOCCUS-AUREUS
CHARACTERIZATION OF AN ISOGENIC SET OF METHICILLIN-RESISTANT AND SUSCEPTIBLE MUTANTS OF STAPHYLOCOCCUS-AUREUS
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DOI：
10.1007/bf02013308
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发表时间：
1986-12-01
影响因子：
4.5
通讯作者：
KAYSER, FH
中科院分区：
文献类型：
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作者：
BERGERBACHI, B;STRASSLE, A;KAYSER, FH
在耐甲氧西林菌株中存在一种低亲和力的青霉素结合蛋白（PBP2”），但在金黄色葡萄球菌等基因敏感菌株中不存在，这种蛋白负责甲氧西林耐药的表型表达。已知控制甲氧西林耐药的附加因子位于染色体插入位点(对应::Tn551)。分析了该因素对PBP2合成的影响。从携带甲氧西林耐药因子的金黄色葡萄球菌菌株和.OMEGA.2003衍生的等基因甲氧西林敏感和耐药突变体（chr::Tn551）插入位点通过种群分析和Tn551插入位点周围DNA的限制性内切分析进行了鉴定，并测定了它们的PBP2”含量。耐甲氧西林和敏感突变体产生的PBP2水平与原始耐甲氧西林亲本相似。这种ppb2的存在与原始的耐甲氧西林亲本相似。因此，这种ppb2的存在可能不是甲氧西林耐药性表型表达的唯一原因。附加因子的性质和作用部位由。omega .2003确定（chr::Tn551）控制甲氧西林耐药表达的位点仍然未知。
A low affinity penicillin binding protein (PBP2'') present in methicillin resistant strains but absent in isogenic sensitive stains of Staphylococcus aureus is responsible for the phenotypic expression of the methicillin resistance An addition factor controlling methicillin resistance is known to map on the chromosome at the insertion site .OMEGA.2003 (chr::Tn551). The influence of this factor on PBP2'' synthesis was analysed. Isogenic methicillin sensitive and resistant mutants derived from a Staphylococcus aureus strain carrying methicillin resistance factors and the .OMEGA.2003 (chr::Tn551) insertion were characterized by population analysis and restriction analysis of the DNA surrounding the Tn551 insertion site, and their PBP2'' content was determined. Both methicillin resistant and sensitive mutants produced levels of PBP2'' similar to those of the original methicillin resistant parent. The mere presence of this PBP2'' similar to those of the original methicillin resistant parent. The mere presence of this PBP2'' could therefore not be solely responsible for the phenotypic expression of methicillin resistance. The nature and site of action of the additional factor determined by the .OMEGA.2003 (chr::Tn551) site controlling expression of methicillin resistance still remains unknown.