Salinity-induced subcellular accumulation of H2O2 in leaves of rice
Salinity-induced subcellular accumulation of H2O2 in leaves of rice
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DOI：
10.1007/s00709-011-0280-7
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发表时间：
2011-04
期刊：
影响因子：
2.9
通讯作者：
K. Yamane;M. Taniguchi;H. Miyake
中科院分区：
文献类型：
--
作者：
K. Yamane;M. Taniguchi;H. Miyake
使用 3,3-二氨基联苯胺和 CeCl3 染色在超微结构水平上检查了水稻叶片中盐诱导的 H2O2 积累的定位。当 3 周龄的水稻植株受到 100 mM NaCl 影响 14 天时，观察到类囊体肿胀和类囊体膜破坏。在用 NaCl 处理的叶子的叶绿体中也观察到 H2O2 积累。 3,3-二氨基联苯胺和CeCl3的电子致密产物主要在类囊体肿胀周围观察到。黑暗条件下叶绿体中未观察到 H2O2 积累和任何超微结构变化。此外，抗坏血酸处理抑制了 H2O2 积累和叶绿体超微结构的变化。这些结果表明，盐度下光诱导产生过量的 H2O2 是导致叶绿体超微结构变化的原因。在光照而非黑暗条件下，线粒体、过氧化物酶体、质膜和细胞壁中也观察到 H2O2 积累，表明这些细胞器也是 H2O2 的来源，并且在盐度下其产生依赖于光。
The localization of salt-induced H2O2accumulation in the leaves of rice was examined using 3,3-diaminobenzidine and CeCl3staining at ultrastructure level. When the 3-week-old rice plants were affected by 100 mM NaCl for 14 days, the swelling of thylakoids and the destruction of thylakoid membranes were observed. H2O2accumulation was also observed in the chloroplast of the leaf treated with NaCl. The electron dense products of 3,3-diaminobenzidine and CeCl3were mainly observed especially around the swelling of thylakoids. H2O2accumulation and any ultrastructural changes were not observed in the chloroplasts under dark condition. Furthermore, treatment with ascorbic acid suppressed both H2O2accumulation and the changes in chloroplast ultrastructure. These results suggest that light-induced production of excess H2O2under salinity is responsible for the changes in chloroplast ultrastructure. H2O2accumulation was also observed in the mitochondria, peroxisomes, plasma membrane, and cell walls under light but not dark, suggesting that these organelles are also the source of H2O2and the production is light dependent under salinity.