Phosphorus metabolites and the distribution of cell cycle phase of RIF-1 tumors in response to 14 Gy irradiation.
Phosphorus metabolites and the distribution of cell cycle phase of RIF-1 tumors in response to 14 Gy irradiation.
复制标题
14 Gy 照射下 RIF-1 肿瘤的磷代谢物和细胞周期时相分布。
DOI：
10.1002/mrm.1910160309
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发表时间：
1990
影响因子：
3.3
通讯作者：
Shin,KH
中科院分区：
文献类型：
--
作者：
Majors,AW;Ng,TC;Karalis,IM;Edinger,MG;Tubbs,RR;Higgins,PD;Shin,KH
对40个经14戈伊X射线照射的RIF-1小鼠肿瘤进行了DNA细胞周期分布和体内31 P NMR光谱的同时测量。观察到二倍体和四倍体肿瘤群体。以G2/M期四倍体细胞数与总细胞数的比值作为G2/M期阻滞细胞的函数。G2/M群体在照射后32 h达到最大值，到72 h下降到对照值，而无机磷酸盐与β-核苷酸三磷酸盐的比率在32 h显著下降，并在照射后72 h保持显著低于对照。PME、PDE、PCr和pH的测量结果在任何时间点均未显示显着变化。未观察到宿主细胞群的显著变化。由于测量的G2/M群体从未增加到超过总细胞群体的3%，在31 P NMR谱中观察到的变化不仅仅是不同细胞相群体的NMR谱可能存在差异的结果，而是更可能是由于大多数细胞的代谢特征或环境的变化。出版社：Academic Press，Inc.
Simultaneous measurements of DNA cell phase cycle distributions andin vivo31P NMR spectroscopy were performed on 40 RIF‐1 murine tumors irradiated with 14 Gy of X‐radiation. Diploid and tetraploid tumor populations were observed. The cells blocked in G2/M phase were measured as a function of the ratios of tetraploid cell number inG2/ Mphase versus total cell population measured. TheG2/Mpopulation reached a maximum at 32 h post irradiation, dropping to control values by 72 h, while the ratio of inorganic phosphate to β‐nucleotide triphosphate dropped significantly at 32 h and remained significantly lower than control up to 72 h post irradiation. Measurements of PME, PDE, PCr, and pH showed no significant variations at any time point. No significant change in host cell population could be observed. Since the measuredG2/Mpopulation never increased to more than 3% of the total cell population, the change observed in the31P NMR spectra were not simply the result of possible differences in NMR profiles of the different cell phase populations but were more likely due to a change in the metabolic characteristics or environment of a majority of the Cells. © 1990 Academic Press, Inc.