Lariatins, antimycobacterial peptides produced by Rhodococcus sp K01-B0171, have a lasso structure
Lariatins, antimycobacterial peptides produced by Rhodococcus sp K01-B0171, have a lasso structure
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DOI：
10.1021/ja056780z
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发表时间：
2006-06-14
影响因子：
15
通讯作者：
Omura, Satoshi
中科院分区：
文献类型：
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作者：
Iwatsuki, Masato;Tomoda, Hiroshi;Omura, Satoshi
从红球菌K 01-B 0171的培养液中分离得到两种抗分枝杆菌活性物质lariatins A和B。其结构通过光谱分析和先进的蛋白质化学方法被阐明为独特的环肽，其由18和20个L-氨基酸残基组成，在Glu 8的γ-羧基和Gly 1的α-氨基之间具有内部连接。通过动态模拟退火方法对Lariatin A的三维结构进行了研究，结果表明，Lariatin A的尾段（Trp 9-Pro 18）通过环段（Gly 1-Glu 8）形成“套索”结构。
Two antimycobacterial agents, lariatins A and B, were isolated from the culture broth of Rhodococcus sp. K01-B0171. Their structures were elucidated by spectral analysis and advanced protein chemical methods to be unique cyclic peptides, which consist of 18 and 20 L-amino acid residues with an internal linkage between the gamma-carboxyl group of Glu8 and the alpha-amino group of Gly1. The three-dimensional structure of lariatin A deduced from NMR data by dynamical simulated annealing method indicates that the tail segment ( Trp9-Pro18) passes through the ring segment ( Gly1-Glu8) to form a 'lasso' structure.