Magnification of rRNA gene number in a Neurospora crassa strain with a partial deletion of the nucleolus organizer.
Magnification of rRNA gene number in a Neurospora crassa strain with a partial deletion of the nucleolus organizer.
复制标题
核仁组织体部分缺失的粗糙脉孢菌菌株中 rRNA 基因数量的放大。
DOI：
10.1007/bf00327592
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发表时间：
1986
期刊：
影响因子：
1.6
通讯作者：
Rodland,KD
中科院分区：
文献类型：
--
作者：
Russell,PJ;Rodland,KD
粗粒eurospora translocation株（IL→VL）OY321与正常序列杂交的部分子代。粗株是核仁组织器部分缺失的重复菌株。尽管有缺失，这些子代仍能存活并产生功能性核仁。在这些缺失的后代中，rRNA基因数量的定量显示rRNA基因的显著缺失，下降到亲本野生型水平的60%。最初，所有这些减少核仁组织者（RNO）菌株都表现出生长管中菌丝伸长率的降低。经过几个营养生长周期后，一些后代恢复到正常生长表型，并且rRNA基因数量也增加到与野生型相近。
Some progeny from crosses between theNeurospora crassatranslocation strainT(IL→ VL)OY321 and normal sequenceN. crassastrains are duplication strains with a partial deletion of the nucleolus organizer. Despite the deletion, these progeny are viable and produce a functional nucleolus. Quantification of rRNA gene number in these deletion progeny demonstrated a significant loss of rRNA genes, down to 60% of the parental wild-type level. Initially, all of these reduced nucleolus organizer (RNO) strains demonstrated a reduction in the rate of mycelial elongation in growth tubes. After several vegetative growth cycles some progeny reverted to the normal growth phenotype, and also showed an increase in the number of rRNA genes to approximately that of the wild type.