LABORATORY IDENTIFICATION OF ADENOVIRUSES ASSOCIATED WITH GASTROENTERITIS IN CANADA FROM 1983 TO 1986
LABORATORY IDENTIFICATION OF ADENOVIRUSES ASSOCIATED WITH GASTROENTERITIS IN CANADA FROM 1983 TO 1986
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DOI：
10.1128/jcm.28.7.1525-1529.1990
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发表时间：
1990-07-01
影响因子：
9.4
通讯作者：
BROWN, M
中科院分区：
文献类型：
--
作者：
BROWN, M
对1983~1986年间收集的143份粪便标本进行了电子显微镜检查，对与胃肠炎相关的腺病毒血清型进行了分析。根据病毒DNA的SmaI裂解图谱和用特异性抗血清中和的方法对140份标本中的病毒分离株进行了分型。其中以腺病毒31型(Ad31)、AD40(16.9%)和AD41(38%)为主，合计占分离株的70%以上。其余的病毒分离株分别为Ad1、2、3、5、7和12。DNA限制性内切酶分析比血清中和法更好地鉴定粪便标本中的肠道腺病毒血清型。HindIII裂解鉴定出四个AD41变异体，没有一个具有与原型株Tak相同的HindIII限制性内切酶图谱。在研究期间，AD40的发病率总体上呈下降趋势，而AD41的发病率却有所上升，而AD31的发病率相对稳定。
A retrospective analysis of adenovirus serotypes associated with gastroenteritis involved the examination of 143 stool specimens collected between 1983 and 1986 from symptomatic patients whose stools were positive for adenovirus by electron microscopy. The virus isolates obtained from 140 of the specimens were typed according to the SmaI cleavage pattern of the viral DNA and by neutralization with specific antisera. The predominant types were adenovirus type 31 (Ad31) Ad40 (16.9%), and Ad41 (38%), which together accounted for more than 70% of the isolates. The remaining virus isolates were typed as Ad1, 2, 3, 5, 7, and 12. DNA restriction analysis proved to be better than serum neutralization for identification of the enteric adenovirus serotypes in stool specimens. HindIII cleavage identified four Ad41 variants, none of which had a HindIII restriction pattern identical to that of the protype strain Tak. Over the time period of the study, the incidence of Ad40 showed an overall decrease accompanied by an increased incidence of Ad41, while the incidence of Ad31 was relatively stable.