Identification and characterization of ecologically significant prokaryotes in the sediment of freshwater lakes: molecular and cultivation studies.
Identification and characterization of ecologically significant prokaryotes in the sediment of freshwater lakes: molecular and cultivation studies.
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DOI：
10.1111/j.1574-6976.2000.tb00559.x
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发表时间：
2000-12
影响因子：
11.3
通讯作者：
Stefan Spring;R. Schulze;Jörg Overmann;K. Schleifer
中科院分区：
文献类型：
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作者：
Stefan Spring;R. Schulze;Jörg Overmann;K. Schleifer
这篇综述的目的是解释最近的研究，其中使用分子方法来识别和表征湖泊沉积物和相关栖息地中的原核生物。在第一部分的研究中，基于湖间栖息地中原核生物的系统发育多样性进行了总结。在本综述的第二部分中，各种独立于培养的方法在不同的沉积物细菌表征中的应用被形态明显，无色的硫细菌体现了体现。最后，描述了传统和最近开发的方法，这些方法可用于将微生物种群的功能与其识别联系起来。讨论了这些方法研究湖泊沉积物的潜力，以便为未来在该栖息地进行研究的观点。
The aim of this review is to interpret recent studies in which molecular methods were used to identify and characterize prokaryotes in lake sediments and related habitats. In the first part studies based on the phylogenetic diversity of prokaryotes found in lacustrine habitats are summarized. The application of various cultivation-independent methods for the characterization of distinct groups of sediment bacteria is exemplified with morphologically conspicuous, colorless sulfur bacteria in the second part of this review. Finally, traditional and recently developed methods are described which could be used for linking the function of microbial populations with their identification. The potential of these approaches for the study of lake sediments is discussed in order to give a perspective for future studies in this habitat.