Purification and characterization of the DNA untwisting enzyme from rat liver.
Purification and characterization of the DNA untwisting enzyme from rat liver.
复制标题
大鼠肝脏 DNA 解旋酶的纯化和表征。
DOI：
10.1021/bi00666a014
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发表时间：
1976
期刊：
影响因子：
2.9
通讯作者：
B. Mcconaughy
中科院分区：
文献类型：
--
作者：
J. Champoux;B. Mcconaughy
DNA 解旋酶已从大鼠肝细胞核中纯化了约 300 倍。通过十二烷基硫酸钠-丙烯酰胺凝胶电泳判断蛋白质纯度大于90%。天然酶的分子量在 64 000 至 68 000 之间，由单条多肽链组成。有证据表明该蛋白质可以发挥催化作用。与最纯级分相关的微量核酸内切酶活性可能是污染物，也可能是由于 DNA 解旋酶本身的作用所致。
The DNA untwisting enzyme has been purified approximately 300-fold from rat liver nuclei. The protein is greater than 90% pure as judged by sodium dodecyl sulfate-acrylamide gel electrophoresis. The native enzyme has a molecular weight between 64 000 and 68 000 and is composed of a single polypeptide chain. Evidence is presented that the protein can act catalytically. A trace amount of endonuclease activity associated with the most pure fraction could be a contaminant or it could be due to the action of the DNA untwisting enzyme itself.