Resistance Risk and Novel Resistance-Related Point Mutations in Target Protein PiORP1 of Fluoxapiprolin in Phytophthora infestans
Resistance Risk and Novel Resistance-Related Point Mutations in Target Protein PiORP1 of Fluoxapiprolin in Phytophthora infestans
复制标题
致病疫霉氟氧哌丙林靶蛋白PiORP1的耐药风险及新的耐药相关点突变
DOI：
10.1021/acs.jafc.1c08199
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发表时间：
2022-04-27
影响因子：
6.1
通讯作者：
Liu，Xili
中科院分区：
文献类型：
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作者：
Li，Chengcheng;Liu，Xiaofei;Liu，Xili
氟沙匹prolin是一种新型的氧甾醇结合蛋白抑制剂（OSBPI），对植物病原卵菌具有良好的抑制活性。其对疫霉的抗性风险及机制尚不清楚。在目前的研究中，103P的敏感性。对氟沙匹prolin的感染呈单峰分布，平均ec50值为0.00035 μg/mL。通过对PiORP1进行杀菌剂适应化，获得了抗性因子在14 ~ 1000以上的4种抗性突变体，以及PiORP1中S768I+N837I、S768I+L860I、S768I和I877F点突变体。突变体的适应度与亲本分离物相似或低于亲本分离物。氟沙匹prolin与oxathiapiprolin交叉耐药呈阳性。在p中证实了点突变。利用CRISPR/Cas9基因组编辑系统进行sojaehomologue定位。含有S768I+N837I或S768I+L860I的变压器具有较高的氟沙匹prolin电阻（RF > 1000）。综上所述，p。对氟沙匹普罗林的耐药程度中等，新型点突变型S768I+N837I或S768I+L860I可引起p较高的氟沙匹普罗林耐药。5种。
Fluoxapiprolin is a new oxysterol binding protein inhibitor (OSBPI), which showed excellent inhibitory activity to plant pathogenic oomycetes. Its resistance risk and mechanism inPhytophthora infestansare unclear. In the current study, the sensitivities of 103P. infestansisolates to fluoxapiprolin were investigated, and a unimodal distribution with a mean EC50value of 0.00035 μg/mL was observed. Four types of resistant mutants, with a resistance factor from 14 to more than 1000, and point mutations S768I+N837I, S768I+L860I, S768I, and I877F in PiORP1, were acquired using fungicide adaption. The fitness of the mutants was similar to or lower than that of the corresponding parental isolate. Positive cross-resistance was detected between fluoxapiprolin and oxathiapiprolin. The point mutations were verified inP. sojaehomologue positions using the CRISPR/Cas9 genome editing system. Transformants containing S768I+N837I or S768I+L860I, showed high fluoxapiprolin resistance (RF > 1000). In conclusion, the risk ofP. infestansresistance to fluoxapiprolin is moderate, and novel point mutation types S768I+N837I or S768I+L860I could cause high fluoxapiprolin resistance inP. infestans.