Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite.
Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite.
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DOI：
10.1039/c4sc03983a
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发表时间：
2015-05-01
期刊：
影响因子：
8.4
通讯作者：
James TD
中科院分区：
文献类型：
--
作者：
Sun X;Lacina K;Ramsamy EC;Flower SE;Fossey JS;Qian X;Anslyn EV;Bull SD;James TD
利用芳香族硼酸与茜素红S(ARS)的自组装，研制了一种选择性检测过氧亚硝酸根的新型化学传感器。利用芳香族硼酸与茜素红S(ARS)的自组装，研制了一种选择性检测过氧亚硝酸根的新型化学传感器。用苯基硼酸(PBA)、苯并硼氧杂多酚(BBA)和2-(N，N-二甲氨基甲基)苯基硼酸(NBA)与ARS结合形成络合物探针。特别是，具有高结合亲和力的ARS-NBA体系可以较好地在过氧化氢和其他ROS/RNS上与过氧亚硝酸根反应，这是因为通过溶剂插入的B-N相互作用得到了硼的保护。我们的简单系统对过氧亚硝酸根产生可见的比色变化和开关荧光响应。通过耦合导致指示剂位移的化学反应，我们开发了一种新的传感策略，在此称为RIA(基于反应的指示剂置换分析)。
Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N,N-dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS–NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B–N interaction. Our simple system produces a visible colorimetric change and on–off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reaction-based Indicator displacement Assay).