Surface modification of interconnected porous scaffolds
Surface modification of interconnected porous scaffolds
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DOI：
10.1002/jbm.a.30367
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发表时间：
2005-07-01
影响因子：
4.9
通讯作者：
Ma, PX
中科院分区：
文献类型：
--
作者：
Liu, XH;Won, YJ;Ma, PX
支架的表面性质对细胞的黏附和生长起着重要的作用。可生物降解的聚α-羟基酸作为组织工程的支架材料已被广泛应用，但缺乏官能团是其局限性。在本工作中，通过一种新的包埋工艺，成功地将明胶固定在聚α-羟基酸薄膜和多孔支架的表面。采用衰减全反射傅里叶变换红外光谱(ATR-FTIR)、X射线光电子能谱(XPS)和接触角测量等手段对样品的表面组成和性能进行了表征。通过改变溶剂组成、浸泡时间、溶液中明胶的浓度和化学交联剂来实现对明胶包埋量的控制。包埋明胶的量随混合溶剂中二氧六环/水的比例增加而增加。物理包埋后的化学交联显著增加了明胶在聚α-羟基酸表面的滞留量。成骨细胞在这些薄膜和支架上培养。表面修饰显著改善了细胞的附着和增殖。细胞接种后4h和1d，表面修饰膜和支架上的细胞数明显高于对照组。体外培养4周时，成骨细胞在表面修饰支架上的增殖率明显高于对照组。表面修饰支架上的胶原纤维和其他细胞分泌物比对照支架上沉积的多。这种新颖的表面处理策略为组织工程提供了一种方便、通用的方法来修饰三维支架的表面性质，从而促进细胞的黏附和增殖。(C)2005年Wiley期刊，Inc.生物医学杂志74A：84-91,2005年。
Surface properties of scaffolds play an important role in cell adhesion and growth. Biodegradable poly(alpha-hydroxy acids) have been widely used as scaffolding materials for tissue engineering; however, the lack of functional groups is a limitation. In this work, gelatin was successfully immobilized onto the surface of poly(a-hydroxy acids) films and porous scaffolds by a new entrapment process. The surface composition and properties were examined using attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectra (XPS), and contact angle measurements. Control over the amount of entrapped gelatin was achieved by varying the solvent composition, the duration of soaking, the concentration of gelatin in solution, and chemical crosslinking. The amount of entrapped gelatin increased with the ratio of dioxane/water in the solvent mixture used. Chemical crosslinking after physical entrapment considerably increased the amount of retained gelatin on the surface of poly(alpha-hydroxy acids). Osteoblasts were cultured on these films and scaffolds. The surface modification significantly improved cell attachment and proliferation. Cell numbers on the surface-modified films and scaffolds were significantly higher than those on controls 4 h and I day after cell seeding. The osteoblasts showed higher proliferation on surface-modified scaffolds than on the control during 4 weeks of in vitro cultivation. More collagen fibers and other cell secretions were deposited on the surface-modified scaffolds than on the control scaffolds. This novel surface treatment strategy provides a convenient and universal way to modify the surface properties of three-dimensional scaffolds and thus promote cell adhesion and proliferation for tissue engineering. (c) 2005 Wiley Periodicals, Inc. J Biomed Mater Res 74A: 84-91, 2005.