MicroRNA expression profiling in the prefrontal cortex of individuals affected with schizophrenia and bipolar disorders.
MicroRNA expression profiling in the prefrontal cortex of individuals affected with schizophrenia and bipolar disorders.
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精神分裂症和躁郁症患者前额皮质中的 MicroRNA 表达谱。
DOI：
10.1016/j.schres.2010.07.002
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发表时间：
2010-12
影响因子：
4.5
通讯作者：
Vladimirov, Vladimir I.
中科院分区：
文献类型：
--
作者：
Kim, Albert H.;Reimers, Mark;Mahera, Brion;Williamson, Vernell;McMichael, Omari I.;McClay, Joseph L.;van den Oord, Edwin J. C. G.;Riley, Brien P.;Kendler, Kenneth S.;Vladimirov, Vladimir I.
microRNA（miRNAs）是一类在mRNA和蛋白质水平上负调控基因表达的非编码小RNA。鉴定的miRNA的数量正在迅速增长，并且大约三分之一在脑中表达，在脑中它们已被证明影响神经元分化、突触体复合体定位和突触可塑性，所有这些功能都被认为在精神分裂症中被破坏。在这里，我们研究了667个miRNAs（miRBase v.13）在精神分裂症（SZ，N=35）和双相情感障碍（BP，N=35）的个人使用实时PCR为基础的Taqman低密度阵列（TLDA）的前额叶皮层的表达。经过广泛的QC步骤后，最终分析中包括441个miRNA。在FDR为10%时，22个miRNAs被鉴定为在病例和对照之间差异表达，7个在SZ中失调，15个在BP中失调。使用计算机模拟靶基因预测程序，发现22种miRNA靶向包含在神经发育，行为以及SZ和BP疾病发展中过度代表的网络中的脑特异性基因。在初步尝试证实这些预测中的一些，我们调查了hsa-mir-34 a，-132和-212的表达与其预测的基因靶点之间的相关程度。在SMRI样品中测量酪氨酸羟化酶（TH）、磷酸葡萄糖酸脱氢酶（PGD）和代谢型谷氨酸受体3（GRM 3）的mRNA表达。Hsa-miR-132和-212与TH（p=0.0001和0.0017）和PGD（分别为p=0.0054和0.017）呈负相关。
MicroRNAs (miRNAs) are a large family of small non-coding RNAs which negatively control gene expression at both the mRNA and protein levels. The number of miRNAs identified is growing rapidly and approximately one-third is expressed in the brain where they have been shown to affect neuronal differentiation, synaptosomal complex localization and synapse plasticity, all functions thought to be disrupted in schizophrenia. Here we investigated the expression of 667 miRNAs (miRBase v.13) in the prefrontal cortex of individuals with schizophrenia (SZ, N=35) and bipolar disorder (BP, N=35) using a real-time PCR-based Taqman Low Density Array (TLDA). After extensive QC steps, 441 miRNAs were included in the final analyses. At a FDR of 10%, 22 miRNAs were identified as being differentially expressed between cases and controls, 7 dysregulated in SZ and 15 in BP. Using in silico target gene prediction programs, the 22miRNAs were found to target brain specific genes contained within networks overrepresented for neurodevelopment, behavior, and SZ and BP disease development. In an initial attempt to corroborate some of these predictions, we investigated the extent of correlation between the expressions of hsa-mir-34a, -132 and -212 and their predicted gene targets. mRNA expression of tyrosine hydroxylase (TH), phosphogluconate dehydrogenase (PGD) and metabotropic glutamate receptor 3 (GRM3) was measured in the SMRI sample. Hsa-miR-132 and -212 were negatively correlated with TH (p=0.0001 and 0.0017) and with PGD (p=0.0054 and 0.017, respectively).
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DOI：
10.1002/ajmg.b.30291
发表时间：
2006-06-05
影响因子：
2.8
作者：
Bajestan, Sepideh N.;Sabouri, Amir H.;Osame, Mitsubiro
通讯作者：
Osame, Mitsubiro
影响因子：
4.1
作者：
Beitzinger, Michaela;Peters, Lasse;Meister, Gunter
通讯作者：
Meister, Gunter
影响因子：
30.8
作者：
Emamian, ES;Hall, D;Gogos, JA
通讯作者：
Gogos, JA
大脑表达的microRNA与精神分裂症病因有关。
影响因子：
3.7
作者：
Hansen, Thomas;Olsen, Line;Lindow, Morten;Jakobsen, Klaus D.;Ullum, Henrik;Jonsson, Erik;Andreassen, Ole A.;Djurovic, Srdjan;Melle, Ingrid;Agartz, Ingrid;Hall, Hakan;Timm, Sally;Wang, August G.;Werge, Thomas
通讯作者：
Werge, Thomas
影响因子：
14.9
作者：
Griffiths-Jones S;Grocock RJ;van Dongen S;Bateman A;Enright AJ
通讯作者：
Enright AJ