MULTIPLE SIGNALING SYSTEMS CONTROLLING EXPRESSION OF LUMINESCENCE IN VIBRIO-HARVEYI - SEQUENCE AND FUNCTION OF GENES ENCODING A 2ND SENSORY PATHWAY
MULTIPLE SIGNALING SYSTEMS CONTROLLING EXPRESSION OF LUMINESCENCE IN VIBRIO-HARVEYI - SEQUENCE AND FUNCTION OF GENES ENCODING A 2ND SENSORY PATHWAY
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DOI：
10.1111/j.1365-2958.1994.tb00422.x
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发表时间：
1994-07-01
影响因子：
3.6
通讯作者：
SILVERMAN, MR
中科院分区：
文献类型：
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作者：
BASSLER, BL;WRIGHT, M;SILVERMAN, MR
哈维氏弧菌发光的密度依赖性表达受培养基中细胞外信号分子（自诱导物）浓度的调节。一个信号响应系统由luxL，M，N基因座编码。luxL和luxM基因是产生自诱导物（可能是β-羟基丁酰基高丝氨酸内酯）所必需的，而luxN基因是对该自诱导物的应答所必需的。LuxL，M和N突变体的表型分析表明，一个额外的信号响应系统也控制密度传感。我们在这里报告的识别，克隆和分析的luxP和luxQ，编码所需的第二密度传感系统的功能。luxP和luxQ缺陷的突变体对第二种自诱导物物质的反应有缺陷。与LuxN一样，LuxQ类似于双组分信号转导蛋白家族的成员，并且包含组氨酸蛋白激酶和反应调节结构域。信号突变体表型的分析表明，至少有两个单独的信号响应途径，收敛到调节的发光哈维氏弧菌的表达。
Density-dependent expression of luminescence in Vibrio harveyi is regulated by the concentration of extracellular signal molecules (autoinducers) in the culture medium. One signal-response system is encoded by the luxL,M,N locus. The luxL and luxM genes are required for the production of an autoinducer (probably beta-hydroxybutryl homoserine lactone), and the luxN gene is required for the response to that autoinducer. Analysis of the phenotypes of LuxL,M and N mutants indicated that an additional signal-response system also controls density sensing. We report here the identification, cloning and analysis of luxP and luxQ, which encode functions required for a second density-sensing system. Mutants with defects in luxP and luxQ are defective in response to a second autoinducer substance. LuxQ, like LuxN, is similar to members of the family of two-component, signal transduction proteins and contains both a histidine protein kinase and a response regulator domain. Analysis of signalling mutant phenotypes indicates that there are at least two separate signal-response pathways which converge to regulate expression of luminescence in V. harveyi.