Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus
Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus
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DOI：
10.1007/s11262-010-0524-1
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发表时间：
2010-12-01
期刊：
影响因子：
1.6
通讯作者：
Chen, Jian-ping
中科院分区：
文献类型：
--
作者：
Mo, Xiao-han;Chen, Zheng-bin;Chen, Jian-ping
烟草丛顶病（Tobacco bushy top disease，TBTV）是由烟草丛顶病毒（tobacco bushy top virus，TBTV）引起的一种烟草丛顶病毒病，它依赖于烟草脉扭曲病毒（tobacco vein-distorting virus，TVDV）作为辅助病毒，通过蚜虫传播烟草丛顶病毒。从感病烟草植株上分离纯化了等距病毒粒子，并在实验蚜虫传毒后复制了感病症状。从病毒RNA克隆的RT-PCR产物中确定TVDV的全基因组。它有5，920个核苷酸（nt）长，具有典型的Polerovirus属成员的六个主要开放阅读框架（ORF）。序列比较表明，它不同于任何其他物种的属，这是证实了RdRp和外壳蛋白的系统发育分析。纯化的病毒粒子的SDS-PAGE分析得到约26和59 kDa的两条蛋白带，这两条蛋白带在Western印迹中与原核表达的TVDV CP产生的抗血清强烈反应，表明TVDV CP的两种形式是衣壳的唯一蛋白组分。
Tobacco bushy top disease is caused by tobacco bushy top virus (TBTV, a member of the genus Umbravirus) which is dependent on tobacco vein-distorting virus (TVDV) to act as a helper virus encapsidating TBTV and enabling its transmission by aphids. Isometric virions from diseased tobacco plants were purified and disease symptoms were reproduced after experimental aphid transmission. The complete genome of TVDV was determined from cloned RT-PCR products derived from viral RNA. It was 5,920 nucleotides (nts) long and had the six major open reading frames (ORFs) typical of a member of the genus Polerovirus. Sequence comparisons showed that it differed significantly from any of the other species in the genus and this was confirmed by phylogenetic analyses of the RdRp and coat protein. SDS-PAGE analysis of purified virions gave two protein bands of about 26 and 59 kDa both of which reacted strongly in Western blots with antiserum produced to prokaryotically expressed TVDV CP showing that the two forms of the TVDV CP were the only protein components of the capsid.