Growth of Ehrlichia canis, the causative agent of canine monocytic ehrlichiosis, in vector and non-vector ixodid tick cell lines
Growth of Ehrlichia canis, the causative agent of canine monocytic ehrlichiosis, in vector and non-vector ixodid tick cell lines
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DOI：
10.1016/j.ttbdis.2016.01.013
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发表时间：
2016-01-01
影响因子：
3.2
通讯作者：
Bell-Sakyi, Lesley
中科院分区：
文献类型：
--
作者：
Ferrolho, Joana;Simpson, Jennifer;Bell-Sakyi, Lesley
犬单核细胞埃立克体病是由犬埃立克体引起的，犬埃立克体是一种感染循环单核细胞的小型革兰氏阴性球状细菌。这种疾病是由棕色狗蜱Rhipicephalus s.l.传播的。并且被认为是世界范围内犬和犬科其它成员的重要传染病。E.犬蜱通常在犬单核细胞-巨噬细胞系DH 82和非载体肩突硬蜱细胞系中体外培养，但不在来源于其天然载体的细胞中培养。在这里，我们报告的感染和有限的传播E。犬蜱在来源于载体R. sanguineus s.l.，并在源自实验载体变异革蜱的细胞系中通过六次传代成功繁殖。此外，利用从I.我们尝试用E.犬埃里希体，作为比较，密切相关的反刍动物埃里希体，反刍动物的心脏水的病原体。Amblyomma和非媒介Dermacentor spp.细胞系对E. canis，但支持E. ruminantium的细胞系，而一些但不是全部的细胞系来源于璃眼蜱属、硬蜱属和扇头蜱属。蜱支持这两种病原体的生长。并对E. DH 82、RSE 8和I.肩胛骨IDE 8细胞。本研究证实E. canis，如E.反刍动物病原体不仅能够在来源于天然和实验蜱载体的细胞系中生长，而且能够在来源于已知不传播该病原体的蜱物种的广泛的其它细胞系中生长。(C)2016作者由爱思唯尔有限公司出版。
Canine monocytic ehrlichiosis is caused by Ehrlichia canis, a small gram-negative coccoid bacterium that infects circulating monocytes. The disease is transmitted by the brown dog tick Rhipicephalus sanguineus s.l. and is acknowledged as an important infectious disease of dogs and other members of the family Canidae worldwide. E. canis is routinely cultured in vitro in the canine monocyte-macrophage cell line DH82 and in non-vector Ixodes scapularis tick cell lines, but not in cells derived from its natural vector. Here we report infection and limited propagation of E. canis in the tick cell line RSE8 derived from the vector R. sanguineus s.l., and successful propagation through six passages in a cell line derived from the experimental vector Dermacentor variabilis. In addition, using bacteria semi-purified from I. scapularis cells we attempted to infect a panel of cell lines derived from non-vector species of the tick genera Amblyomma, Dermacentor, Hyalomma, Ixodes and Rhipicephalus with E. canis and, for comparison, the closely-related Ehrlichia ruminantium, causative agent of heartwater in ruminants. Amblyomma and non vector Dermacentor spp. cell lines appeared refractory to infection with E. canis but supported growth of E. ruminantium, while some, but not all, cell lines derived from Hyalomma, Ixodes and Rhipicephalus spp. ticks supported growth of both pathogens. We also illustrated and compared the ultrastructural morphology of E. canis in DH82, RSE8 and I. scapularis IDE8 cells. This study confirms that E. canis, like E. ruminantium, is able to grow not only in cell lines derived from natural and experimental tick vectors but also in a wide range of other cell lines derived from tick species not known to transmit this pathogen. (C) 2016 The Authors. Published by Elsevier GmbH.