Definition of the uptake mechanism and sub-cellular localisation profile of emissive lanthanide complexes as cellular optical probes
Definition of the uptake mechanism and sub-cellular localisation profile of emissive lanthanide complexes as cellular optical probes
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DOI：
10.1039/c0sc00105h
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发表时间：
2010-01-01
期刊：
影响因子：
8.4
通讯作者：
Parker, David
中科院分区：
文献类型：
--
作者：
New, Elizabeth J.;Congreve, Aileen;Parker, David
为了更好地了解发射性镧系元素络合物作为细胞染色剂或探针的细胞摄取和定位行为，已经进行了一系列实验。出了大量的结构上相关的配合物的特点，最近，一组七个代表性的例子已被详细检查，含有四氮杂苯并菲或氮杂蒽酮为基础的敏化发色团。已经通过显微镜和流式细胞术研究了细胞内定位概况和细胞摄取和排出行为。典型地，最大细胞内浓度为约0.4mM，或约10（9）个复合物/细胞。研究的复合物一般没有毒性，并没有扰乱线粒体膜电位。已经确定了巨胞饮作用的一种常见摄取机制。概括的趋势的行为，和结构-活性关系，并为未来的探针设计的影响进行了讨论。
A series of experiments has been undertaken in order to gain a greater understanding of the cellular uptake and localisation behaviour of emissive lanthanide complexes as cellular stains or probes. Out of a large number of structurally related complexes characterised recently, a set of seven representative examples has been examined in detail, containing either tetraazatriphenylene or azaxanthone-based sensitising chromophores. Intracellular localisation profiles and cellular uptake and egress behaviour have been studied by microscopy and flow cytometry. Typically, the maximum intracellular concentration was of the order of 0.4 mM, or about 10(9) complexes per cell. The complexes studied were generally not toxic and did not perturb the mitochondrial membrane potential. A common uptake mechanism of macropinocytosis has been identified. A generalisation of trends in behaviour, and structure-activity relationships is presented, and the implications for future probe design discussed.