THE ARGININOSUCCINATE LYASE GENE OF CHLAMYDOMONAS-REINHARDTII - AN IMPORTANT TOOL FOR NUCLEAR TRANSFORMATION AND FOR CORRELATING THE GENETIC AND MOLECULAR MAPS OF THE ARG7 LOCUS
THE ARGININOSUCCINATE LYASE GENE OF CHLAMYDOMONAS-REINHARDTII - AN IMPORTANT TOOL FOR NUCLEAR TRANSFORMATION AND FOR CORRELATING THE GENETIC AND MOLECULAR MAPS OF THE ARG7 LOCUS
复制标题
DOI：
10.1002/j.1460-2075.1989.tb08426.x
复制
发表时间：
1989-10-01
期刊：
影响因子：
11.4
通讯作者：
ROCHAIX, JD
中科院分区：
文献类型：
--
作者：
DEBUCHY, R;PURTON, S;ROCHAIX, JD
用四种寡核苷酸探针克隆了莱茵衣藻的琥珀酸溶胞产物（ASL）基因，这些探针对应于ASL多肽序列的高度保守区域。通过部分测序证实了基因的身份。它是独特的，包含几个内含子，并跨越一个区域< 7.8 kb，包括高度重复序列。使用粒子枪，一个可靠的核转化系统已经建立了补充三个突变体的ASL活性与野生型ASL基因。转化体的分析揭示了转化DNA整合到核基因组中的可变模式。先前的工作已经将突变体arg2和arg7中的突变映射到ARG7位点的两端，相距1.0至1.6个重组图单位。我们的转化结果表明，这两个突变位于7.8 kb的区域内。这允许重组图谱和ARG7基因座处的分子图谱的第一相关性，并且表明核基因组的该区域中的高重组频率。
The argininosuccinate lysate (ASL) gene of Chlamydomonas reinhardtii has been cloned using four oligonucleotide probes corresponding to highly conserved regions of the ASL polypeptide sequence. The identity of the gene was confirmed by partial sequencing. It is unique, contains several introns and spans a region < 7.8 kb that includes highly repetitive sequences. Using a particle gun, a reliable nuclear transformation system has been established by complementing three mutants deficient in ASL activity with the wild-type ASL gene. Analysis of the transformants reveals variable patterns of integration of the transforming DNA into the nuclear genome. Previous work has mapped the mutations in the mutants arg2 and arg7 to either end of the ARG7 locus 1.0 to 1.6 recombination map units apart. Our transformation results show that these two mutations are located within a region of 7.8 kb. This allows for the first correlation of the recombination map and the molecular map at the ARG7 locus and indicates a high recombination frequency in this region of the nuclear genome.