Homologous recombination with DNA introduced into mammalian cells.
Homologous recombination with DNA introduced into mammalian cells.
复制标题
与导入哺乳动物细胞的 DNA 进行同源重组。
DOI：
10.1101/sqb.1984.049.01.019
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Kucherlapati,RS
中科院分区：
文献类型：
--
作者：
Smithies,O;Koralewski,MA;Song,KY;Kucherlapati,RS
The G418 R recombinants obtained in our plasmid• plasmid experiments could have arisen via any of a number of homologous recombinational events, some of which are shown schematically in Figure 1. The simplest conceptually is a single crossover within the 500-bp region of homology lying between the two deletions in the neo gene. Such a crossover would generate dimeric plasmids containing both reciprocal recombinants: a full-length and a doubly deleted neo gene. Gene conversion in either direction (or double crossovers) could also generate full-length and doubly deleted neo genes, in this case on monomeric plasmids. A single crossover with gene conversion would generate dimeric plasmids of several types. Our present experiments do not distinguish these and various other possibilities.We have used the plasmid• plasmid system to look for factors that might increase the frequency of homologous recombination leading to an intact neo gene. The most striking is an approximately 10-fold increase (see the lower part of Table 1) obtained when either of the input plasmids is cut at the border of the deleted
