RAPID 7-HOUR FECAL COLIFORM TEST
RAPID 7-HOUR FECAL COLIFORM TEST
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DOI：
10.1128/aem.38.2.229-236.1979
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发表时间：
1979-01-01
影响因子：
4.4
通讯作者：
GELDREICH, EE
中科院分区：
文献类型：
--
作者：
REASONER, DJ;BLANNON, JC;GELDREICH, EE
开发了一种用于检测水中 FC 的 7 小时粪便大肠菌 (FC) 快速测试。该膜过滤测试利用轻度缓冲的乳糖培养基（m-7-h FC 培养基）与敏感的 pH 指示剂系统相结合。在 41.5°C 孵育 7-7.25 小时后，FC 菌落在浅紫色背景下呈现黄色。 FC测试结果比较表明，地表水样品的平均验证FC计数比（7小时FC计数/24小时FC计数）为1.08。未氯化废水的平均 FC 计数比（7 小时 FC 计数/24 小时 FC 计数）范围为 1.95-5.05。 m-7-h FC 培养基中黄色 FC 菌落的验证率平均为 97%。对美国密歇根湖海滨浴场水样进行现场测试的数据显示，未经验证的 7 小时 FC 计数平均为 24 小时 FC 计数的 96%。 7小时FC测试适用于地表水和未氯化污水的检查，可作为饮用水污水或粪便污染检测的紧急测试。
A rapid 7-h fecal coliform (FC) test for the detection of FC in water was developed. This membrane filter test utilizes a lightly buffered lactose-based medium (m-7-h FC medium) combined with a sensitive pH indicator system. FC colonies appeared yellow against a light purple background after incubation at 41.5 C for 7-7.25 h. Comparison of FC test results showed that the mean verified FC count ratio (7-h FC count/24-h FC count) for surface water samples was 1.08. The mean FC count ratio (7-h FC count/24-h FC count) for unchlorinated wastewater ranged from 1.95-5.05. Verification of yellow FC colonies from m-7-h FC medium averaged 97%. Data from field tests on Lake Michigan [USA] bathing beach water samples showed that unverified 7-h FC counts averaged 96% of the 24-h FC counts. The 7-h FC test was suitable for the examination of surface waters and unchlorinated sewage and could serve as an emergency test for detection of sewage or fecal contamination of potable water.