Lymph node conduits transport virions for rapid T cell activation
Lymph node conduits transport virions for rapid T cell activation
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DOI：
10.1038/s41590-019-0342-0
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发表时间：
2019-05-01
影响因子：
30.5
通讯作者：
Hickman, Heather D.
中科院分区：
文献类型：
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作者：
Reynoso, Glennys V.;Weisberg, Andrea S.;Hickman, Heather D.
尽管人们对抗病毒T细胞引发非常感兴趣，但对病毒体在淋巴结（LN）中移动的途径还不完全了解。目前的模型无法解释病毒感染后，病毒感染的细胞如何迅速出现在LN内部。为了更好地理解LN中的病毒粒子运输，我们确定了向小鼠施用牛痘病毒或寨卡病毒后病毒粒子和感染细胞的位置。值得注意的是，LN内部的许多快速感染的细胞邻近LN管道。通过使用共聚焦和电子显微镜，我们清楚地可视化管道内的病毒粒子。在功能上，CD 8（+）T细胞迅速且优先与LN副皮质中被牛痘病毒感染的细胞结合，从而导致LN内部的T细胞活化。这些结果表明，即使是大的病毒体也有可能流过LN导管并感染T细胞区内的树突状细胞以引发CD8（+）T细胞。
Despite intense interest in antiviral T cell priming, the routes by which virions move in lymph nodes (LNs) are imperfectly understood. Current models fail to explain how virus-infected cells rapidly appear within the LN interior after viral infection. To better understand virion trafficking in the LN, we determined the locations of virions and infected cells after administration to mice of vaccinia virus or Zika virus. Notably, many rapidly infected cells in the LN interior were adjacent to LN conduits. Through the use of confocal and electron microscopy, we clearly visualized virions within conduits. Functionally, CD8(+) T cells rapidly and preferentially associated with vaccinia virus-infected cells in the LN paracortex, which led to T cell activation in the LN interior. These results reveal that it is possible for even large virions to flow through LN conduits and infect dendritic cells within the T cell zone to prime CD8(+) T cells.