Polynuclear SmIII Polyamidoamine-Based Dendrimer: A Single Probe for Combined Visible and Near-Infrared Live-Cell Imaging
Polynuclear SmIII Polyamidoamine-Based Dendrimer: A Single Probe for Combined Visible and Near-Infrared Live-Cell Imaging
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DOI：
10.1002/anie.201311028
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发表时间：
2014-03-10
影响因子：
16.6
通讯作者：
Eliseeva, Svetlana V.
中科院分区：
文献类型：
--
作者：
Foucault-Collet, Alexandra;Shade, Chad M.;Eliseeva, Svetlana V.
本文报道了一种发光多核树枝状结构（Sm-III-G3 P-2，3 Nap）的合成，其中8个Sm-III离子被32个2，3-萘酰亚胺发色团敏化。在单一激发波长下，树枝状聚合物复合物在可见光和近红外（NIR）范围内表现出两种类型的发射。Sm-III-G3 P2，3 Nap在孵育24小时后无细胞毒性，最高可达2.5 μ m。的Sm-III为基础的探针被细胞所采取的能力，证实了通过共聚焦显微镜。落射荧光显微镜验证了Sm-III-G3 P-2，3 Nap作为一种多功能探针，能够在可见光或NIR中互换地进行活细胞成像。由于两种排放均来自单一复合物，因此细胞毒性和生物分布本质上相同。在两个光谱范围内区分尖锐的Sm-III信号和自发荧光的可能性增加了分析的可靠性，并降低了人为因素和仪器误差的可能性。
We report herein the synthesis of a luminescent polynuclear dendritic structure (Sm-III-G3P-2,3Nap) in which eight Sm-III ions are sensitized by thirty-two 2,3-naphthalimide chromophores. Upon a single excitation wavelength, the dendrimer complex exhibits two types of emission in the visible and in the near-infrared (NIR) ranges. Sm-III-G3P2,3Nap was non-cytotoxic after 24 h of incubation and up to 2.5 mu m. The ability of the Sm-III-based probe to be taken up by cells was confirmed by confocal microscopy. Epifluorescence microscopy validated Sm-III-G3P-2,3Nap as a versatile probe, capable of performing interchangeably in the visible or NIR for live-cell imaging. As both emissions are obtained from a single complex, the cytotoxicity and biodistribution are inherently the same. The possibility for discriminating the sharp Sm-III signals from autofluorescence in two spectral ranges increases the reliability of analysis and reduces the probability of artifacts and instrumental errors.