AGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals
AGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals
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DOI：
10.1073/pnas.200217597
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发表时间：
2000-10-10
影响因子：
11.1
通讯作者：
Vaucheret, H
中科院分区：
文献类型：
--
作者：
Fagard, M;Boutet, S;Vaucheret, H
转基因DNA的引入可导致与转基因转录序列同源的RNA的特异性降解，这通过在植物中称为转录后基因沉默（PTGS）、在真菌中称为抑制（quelling）和在动物中称为RNA干扰（RNAi）的现象来实现。之前已经证实PTGS。抑制和RNAi需要一组相关蛋白（分别为SCS 2、QDE-1和EGO-1）。在这里，我们报告的分离拟南芥突变体受损的PTGS在Argonaote 1（AGO 1）基因座的影响。AGO 1类似于QDE-2所需的镇压和RDE-1所需的RNAi。对ago 1突变体的测序揭示了PTGS所必需的一个氨基酸，该氨基酸也存在于QDE-2和RDE-1中的高度保守基序中。总之，这些结果证实了这一假设，即这些过程来自一个共同的祖先机制，控制入侵的核酸分子在转录后水平的表达。与rde-1和qde-2突变体不同，ago 1突变体表现出几种发育异常，包括不育。这些结果提高了PTGS的可能性。或至少其部分元件，可能参与植物发育过程中基因表达的调控。
Introduction of transgene DNA may lead to specific degradation of RNAs that are homologous to the transgene transcribed sequence through phenomena named post-transcriptional gene silencing (PTGS) in plants, quelling in fungi, and RNA interference (RNAi) in animals. It was shown previously that PTGS. quelling, and RNAi require a set of related proteins (SCS2, QDE-1, and EGO-1, respectively). Here we report the isolation of Arabidopsis mutants impaired in PTGS which are affected at the Argonaote1 (AGO1) locus. AGO1 is similar to QDE-2 required for quelling and RDE-1 required for RNAi. Sequencing of ago1 mutants revealed one amino acid essential for PTGS that is also present in QDE-2 and RDE-1 in a highly conserved motif. Taken together, these results confirm the hypothesis that these processes derive from a common ancestral mechanism that controls expression of invading nucleic acid molecules at the post-transcriptional level. As opposed to rde-1 and qde-2 mutants, which are viable, ago1 mutants display several developmental abnormalities, including sterility. These results raise the possibility that PTGS. or at least some of its elements, could participate in the regulation of gene expression during development in plants.