Thermodynamically Controlled Self-Assembly of Hierarchically Staggered Architecture as an Osteoinductive Alternative to Bone Autografts
Thermodynamically Controlled Self-Assembly of Hierarchically Staggered Architecture as an Osteoinductive Alternative to Bone Autografts
复制标题
热力学控制的分层交错结构自组装作为自体骨移植物的骨诱导替代方案（IF：18.808）
DOI：
10.1002/adfm.201806445
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发表时间：
2019-03-07
影响因子：
19
通讯作者：
Zhou, Yanheng
中科院分区：
文献类型：
--
作者：
Liu, Yan;Luo, Dan;Zhou, Yanheng
骨诱导合成生物材料可以通过模仿骨的层次结构和表面形态来替代自体移植，以促进宿主细胞的招募和分化。到目前为止，由于体外合成途径的能量变化与体内自然过程的能量变化本质上不同，因此复制骨状交错层级结构一直是具有挑战性的。在这里，在热力学控制下再现了一种骨状的分层交错结构，包括两个步骤：高能聚丙烯酸-钙中间体的制备和由能量下坡过程驱动的胶原间隙区域的选择性矿化。中间能量间隔可以很容易地调整，以确定不同的矿化模式，具有不同的形态和生物功能。与自体骨移植相似，交错结构在体外为干细胞募集和多向分化提供了骨特异的微环境，并在体内通过宿主干细胞归巢诱导骨髓血管形成新骨。这项工作为体外模拟生物矿化过程提供了一个新的视角，为智能生物材料的临床应用提供了概念验证。
Osteoinductive synthetic biomaterials for replacing autografts can be developed by mimicking bone hierarchy and surface topography for host cell recruitment and differentiation. Until now, it has been challenging to reproduce a bone-like staggered hierarchical structure since the energy change underlying synthetic pathways in vitro is essentially different from that of the natural process in vivo. Herein, a bone-like hierarchically staggered architecture is reproduced under thermodynamic control involving two steps: fabrication of a high-energy polyacrylic acid-calcium intermediate and selective mineralization in collagenous gap regions driven by an energetically downhill process. The intermediate energy interval could easily be adjusted to determine different mineralization modes, with distinct morphologies and biofunctions. Similar to bone autografts, the staggered architecture offers a bone-specific microenvironment for stem cell recruitment and multidifferentiation in vitro, and induces neo-bone formation with bone marrow blood vessels by host stem cell homing in vivo. This work provides a novel perspective for an in vitro simulating biological mineralization process and proof of concept for the clinical application of smart biomaterials.