Detection of cholera toxin in seafood using a ganglioside-liposome immunoassay
Detection of cholera toxin in seafood using a ganglioside-liposome immunoassay
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DOI：
10.1007/s00216-007-1551-1
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发表时间：
2008-05-01
影响因子：
4.3
通讯作者：
Durst, Richard A.
中科院分区：
文献类型：
--
作者：
Ahn, Soohyoun;Durst, Richard A.
食品的微生物污染仍然是公共卫生方面的一个主要问题。生物毒素是一类重要的污染物，可引起人类多种疾病。与生物毒素或产毒微生物污染有关的疫情使得开发快速（约20分钟）、灵敏和成本效益高的分析方法变得极为重要。本文介绍了一种敏感的生物测定法的发展，用于检测霍乱毒素（CT）在选定的海鲜样品，使用神经节苷脂掺入脂质体。在本研究中，使用加标不同浓度CT的食品样品进行测定。与先前报道的缓冲系统中的检测限相比，在大多数食品样品中的检测限增加了约10-20倍。然而，食物样品中毒素的最低检测限（CT为8 x 10-3 x 10（3）fg/mL）仍然与先前报告的其他检测方法相当或更低。本研究的结果表明，神经节苷脂脂质体生物检测法可以直接检测毒素在食品样品的现场筛选快速，简单，可靠，而不需要复杂的仪器。
Microbiological contamination of foods continues to be a major concern in public health. Biological toxins are one class of important contaminants that can cause various human diseases. Outbreaks related to contamination by biological toxins or toxin-producing microorganisms have made it extremely important to develop rapid (approximately 20 min), sensitive and cost-effective analytical methods. This paper describes the development of a sensitive bioassay for the detection of cholera toxin (CT) in selected seafood samples, using ganglioside-incorporated liposomes. In this study, the assays were run with food samples spiked with various concentrations of CT. The limit of detection (LOD) increased by a factor of about 10-20 in most food samples, compared with the LOD in the buffer system previously reported. However, the LOD of toxins in food samples (8 x 10-3 x 10(3) fg/mL for CT) was still comparable to, or lower than, that previously reported for other assays. The results from this study demonstrate that the bioassays using ganglioside-liposomes can detect the toxin directly in the field screening of food samples rapidly, simply and reliably, without the need for complex instrumentation.