Meiotic recombination remote from prominent DNA break sites in S. pombe.
Meiotic recombination remote from prominent DNA break sites in S. pombe.
复制标题
减数分裂重组远离粟酒裂殖酵母中显着的 DNA 断裂位点。
DOI：
10.1016/s1097-2765(02)00452-5
复制
发表时间：
2002
期刊：
影响因子：
16
通讯作者：
Smith,GeraldR
中科院分区：
文献类型：
--
作者：
Young,JenniferA;Schreckhise,RandallW;Steiner,WalterW;Smith,GeraldR
DNA断裂与裂殖酵母粟酒裂殖酵母的减数分裂重组密切相关。在所调查的基因组区域中，发现了相距约25至200 kb的显著DNA断裂位点。我们详细检查了染色体I的501 kb区域，发现在arad 50 S突变体中有6个位点，或紧密的位点簇，在这些位点上有大约2%-11%的DNA积累断裂。与显著断裂位点的离散、宽间隔分布相反，无论测试的遗传间隔是否包含无、一个或多个此类位点，该区域中的重组都更均匀地分布（0.7-1.6 cM/10 kb）。我们推断，虽然重组取决于DNA断裂，重组往往发生在远离这些网站（数十个酶），我们讨论的机制，这可能发生。
DNA breakage is intimately associated with meiotic recombination in the fission yeastSchizosaccharomyces pombe. Sites of prominent DNA breakage were found ∼25 to ∼200 kb apart in the genomic regions surveyed. We examined in detail a 501 kb region of chromosome I and found six sites, or tight clusters of sites, at which ∼2%–11% of the DNA accumulated breaks in arad50S mutant. In contrast to the discrete, widely spaced distribution of prominent break sites, recombination in this region was more uniformly distributed (0.7–1.6 cM/10 kb) whether the genetic interval tested contained no, one, or more such sites. We infer that although recombination depends upon DNA breakage, recombination often occurs remote from these sites (tens of kilobases away); we discuss mechanisms by which this may occur.