Polyadenylate metabolism in the nuclei and cytoplasm of Saccharomyces cerevisiae.
Polyadenylate metabolism in the nuclei and cytoplasm of Saccharomyces cerevisiae.
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酿酒酵母细胞核和细胞质中的聚腺苷酸代谢。
DOI：
10.1016/s0021-9258(19)41227-1
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
S. Phillips
中科院分区：
文献类型：
--
作者：
B. Groner;S. Phillips
设计了一种程序，用于在一步中同时分离酿酒酵母 alphas288c 原生质球的细胞核和细胞质。我们检查了这些级分中含有聚腺苷酸聚 (A) 的 RNA 及其合成动力学。核 RNA 在 10 分钟内被 [3H] 腺嘌呤饱和。标记的RNA很快出现在细胞质中，添加[3H]腺嘌呤后2分钟内就超过了标记的核RNA的量。核内含 Poly(A) 的 RNA 约占从标记 15 分钟的原生质球中获得的总细胞含 Poly(A) RNA 的 10%。核内含有poly(A)的RNA不像动物细胞中巨大的异质核RNA那么大。含 Poly(A) RNA 的核和细胞质群体中分子大小的分布非常广泛，核物种的平均大小略大于细胞质物种。从含有 Poly(A) 的酵母 RNA 中获得了三种不同大小类别的 Poly(A)，具有不同的表观动力学特性。它们的电泳迁移率表明分子长度约为 20、40 和 60 个核苷酸（Groner, G.、Hynes, N. 和 Phillips, S. (1975) Biochemistry, 13, 5378-5383）。这些 Poly(A) 类别中的每一种都存在于大和小多聚核糖体的 mRNA 中。
A procedure has been designed for the simultaneous isolation, in a single step, of the nuclei and cytoplasm of Saccharomyces cerevisiae alphas288c spheroplasts. We have examined the polyadenylate poly(A)-containing RNA in these fractions and their kinetics of synthesis. Nuclear RNA saturates with [3H] adenine within 10 min. Labeled RNA appears very quickly in the cytoplasm, exceeding the amount of labeled nuclear RNA within 2 min after the addition of [3H] adenine. Nuclear poly(A)-containing RNA is approximately 10% of the total cellular poly(A)-containing RNA obtained from spheroplasts labeled for 15 min. Nuclear poly(A)-containing RNA is not as large as the giant heterogeneous nuclear RNA of animal cells. The distribution of molecular size in nuclear and cytoplasmic populations of poly(A)-containing RNA is very broad with the average size of the nuclear species being moderately larger than the cytoplasmic species. Three distinct size classes of poly(A), with different apparent kinetic properties, are obtained from yeast poly(A)-containing RNA. Their electrophoretic mobility suggests molecular lengths of approximately 20, 40, and 60 nucleotides (Groner, G., Hynes, N., and Phillips, S. (1975) Biochemistry, 13, 5378-5383). Each of these poly(A) classes are present in the mRNA from large and small polyribosomes.