Expression of CD109 in human cancer
Expression of CD109 in human cancer
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DOI：
10.1038/sj.onc.1207418
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发表时间：
2004-04-29
期刊：
影响因子：
8
通讯作者：
Takahashi, M
中科院分区：
文献类型：
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作者：
Hashimoto, M;Ichihara, M;Takahashi, M
最近报道，人CD 109基因编码糖基-磷脂酰肌醇锚定的糖蛋白，其是含硫酯蛋白的α（2）-巨球蛋白/C3、C4、C5家族的成员。在这项研究中，我们发现，小鼠CD 109基因的表达上调NIH 3 T3细胞表达RET酪氨酸激酶与多发性内分泌肿瘤2B突变。北方印迹分析显示，在正常人和小鼠组织中，CD 109基因仅在睾丸中高水平表达。此外，它在一些人类肿瘤细胞系中高表达，包括鳞状细胞癌和胶质母细胞瘤细胞系，而在神经母细胞瘤和小细胞肺癌细胞系中检测不到。当用定量RT-PCR检测33例人肺细胞癌中CD 109的表达时，在大约一半的鳞状细胞癌中检测到CD 109的显著高表达，而在腺癌、大细胞癌和小细胞癌中没有检测到CD 109的表达。类似地，在17例食管鳞状细胞癌中的9例中观察到CD 109的上调。因此，这些结果表明，CD 109可能是一个有用的分子靶点，为恶性肿瘤，如鳞状细胞癌的新疗法的发展。
It was recently reported that the human CD109 gene encodes a glycosyl-phosphatidylinositol-anchored glycoprotein that is a member of the alpha(2)-macroglobulin/C3, C4, C5 family of thioester-containing proteins. In this study, we found that the expression of mouse CD109 gene was upregulated in NIH3T3 cells expressing RET tyrosine kinase with a multiple endocrine neoplasia 2B mutation. Northern blot analysis showed a high level of expression of the CD109 gene only in the testis in normal human and mouse tissues. In addition, its expression was high in some human tumor cell lines, which included squamous cell carcinoma and glioblastoma cell lines, whereas it was undetectable in neuroblastoma and small-cell lung carcinoma cell lines. When CD109 expression was examined in 33 cases of human lung cell carcinomas by quantitative RT-PCR, a significant high expression of CD109 was detected in about half of squamous cell carcinomas examined, but not in adenocarcinoma, large-cell carcinoma and small-cell carcinoma. Similarly, upregulation of CD109 was observed in nine out of 17 esophageal squamous cell carcinomas. Thus, these results suggested that CD109 might be a useful molecular target for the development of new therapeutics for malignant tumors, such as squamous cell carcinoma.