Dose and dose-rate dependence of DSB-type mutants induced by X-rays or tritium beta-rays: An approach using a hyper-sensitive system.
Dose and dose-rate dependence of DSB-type mutants induced by X-rays or tritium beta-rays: An approach using a hyper-sensitive system.
复制标题
X 射线或氚 β 射线诱导的 DSB 型突变体的剂量和剂量率依赖性：使用超敏感系统的方法。
DOI：
10.1093/rpd/ncac036
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发表时间：
2022
影响因子：
1
通讯作者：
Tauchi H
中科院分区：
文献类型：
--
作者：
Nagashima H;Hayashi Y;Tanimoto;S;Tauchi H
为了评估低水平辐射引发的生物效应，我们建立了一个独特的敏感实验系统来检测体细胞突变。通过使用该系统，我们发现，X射线诱发的突变频率在剂量超过0.15戈伊的统计学意义，并观察到剂量与突变频率的线性关系，剂量超过0.15戈伊。突变谱分析表明，由低于0.1戈伊的X射线剂量产生的突变事件与未辐照对照中观察到的突变事件相似。此外，一个显着的拐点，突变频率和突变谱，被发现在剂量率约11毫戈瑞/天，当细胞培养在含氚水的培养基中。由于诱发辐射型事件在临界剂量或拐点以上呈现出明显的剂量/剂量率依赖性，这些观察结果表明，辐射产生的突变事件可能在阈值剂量率或临界剂量下发生变化。
To evaluate biological effects triggered by low levels of radiation, we established a uniquely sensitive experimental system to detect somatic mutations. By using the system, we found that mutant frequencies induced by X-rays were statistically significant at doses over 0.15 Gy, and a linear dose relationship with the mutant frequency was observed at doses over 0.15 Gy. The mutation spectra analysis revealed that mutation events generated by X-ray doses below 0.1 Gy were similar to those observed in unirradiated controls. In addition, a significant inflection point for both, the mutant frequency and the mutation spectra, was found at dose-rates around 11 mGy/day when cells were cultured in medium containing tritiated water. Because induced radiation-type events presented a clear dose/dose-rate dependency above the critical dose or the inflection point, these observations suggest that mutation events generated by radiation could change at a threshold dose-rate or a critical dose.