Rosbin: A novel homeobox-like protein gene expressed exclusively in round spermatids
Rosbin: A novel homeobox-like protein gene expressed exclusively in round spermatids
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DOI：
10.1095/biolreprod.103.026096
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发表时间：
2004-05-01
影响因子：
3.6
通讯作者：
Nishimune, Y
中科院分区：
文献类型：
--
作者：
Takahashi, T;Tanaka, H;Nishimune, Y
哺乳动物的精子发生是一个复杂的过程，在生精小管内以高度协调的方式发生。为了阐明控制单倍体生殖细胞分化的分子机制，我们从小鼠睾丸的消减cDNA文库中分离了单倍体生殖细胞特异性的cDNA克隆。其中Rosbin基因全长3.2kb，开放阅读框2385个核苷酸，编码795个氨基酸残基的蛋白质。计算机介导的同源搜索显示，它包含一个类似于同源框基因的结构域。Northern印迹分析显示，3.2kb的mRNA仅在雄性生殖细胞中表达。在青春期前的睾丸中没有观察到Rosbin基因的转录，但在第23天后就可以检测到。经Western印迹分析，该基因编码的蛋白质分子量为89 kDa，定位于IV-VIII期单倍体圆形精子细胞的胞核，主要位于生精发生的VII-VIII期。ROSBIN与蛋白激酶A相关，并且很可能被蛋白激酶A磷酸化。我们认为它在单倍体生殖细胞的转录调控中发挥着重要作用。
Mammalian spermiogenesis is a complex process occurring in a highly coordinated fashion within the seminiferous tubules. To elucidate the molecular mechanisms controlling haploid germ cell differentiation, we have isolated haploid germ cell-specific cDNA clones from a subtracted cDNA library of mouse testis. One of these cDNAs, Rosbin, is 3.2 kilobases (kb) long and has an open reading frame of 2385 nucleoticles encoding a putative protein of 795 amino acid residues. A computer-mediated homology search revealed that it contained a domain similar to that of homeobox genes. Northern blot analysis revealed a 3.2-kb mRNA expressed exclusively in male germ cells. Transcription of the Rosbin gene was not observed in prepubertal testis but became detectable after Day 23. By Western blot analysis the protein encoded by this gene had a molecular mass of 89 kDa, expressing specifically in the testis and localized to the nucleus of stages IV-VIII haploid round spermatids, predominantly at stages VII-VIII of spermatogenesis. ROSBIN is associated with and is most likely phosphorylated by protein kinase A. We suggest that it plays an important role in transcriptional regulation in haploid germ cells.