Expression of c-kit and Slug correlates with invasion and metastasis of salivary adenoid cystic carcinoma
Expression of c-kit and Slug correlates with invasion and metastasis of salivary adenoid cystic carcinoma
复制标题
c-kit、Slug表达与唾液腺腺样囊性癌侵袭转移相关
DOI：
10.1016/j.oraloncology.2010.02.001
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发表时间：
2010-04-01
期刊：
影响因子：
4.8
通讯作者：
Chen, Yu
中科院分区：
文献类型：
--
作者：
Tang, Yaling;Liang, Xinhua;Chen, Yu
c-kit在涎腺腺样囊性癌（adenoid cystic carcinoma，ACC）中的高表达是常见的和特异性的，但c-kit的表达与ACC的侵袭转移的关系尚不清楚。最近的研究表明，上皮-间质转化（EMT）转录因子Slug是c-kit信号通路生物学特异性的分子靶点。本研究检测了两种ACC细胞系和121例ACC患者c-kit和Slug的表达，real-time RT-PCR和Western blot结果显示ACC-2和ACC-M细胞系均表达c-kit和Slug mRNA和蛋白。免疫组化结果显示，c-kit和Slug的阳性表达率分别为89.26%（108/121）和71.90%（87/121），c-kit和Slug的表达与ACC的肿瘤部位、TNM分期、组织学类型、神经浸润、局部复发和远处转移有关（P < 0.01）。
The overexpression of c-kit seems to be frequent and specific in salivary adenoid cystic carcinoma (ACC), however, there is little information on correlation between c-kit expression and the invasion and metastasis. Recently, the data showed that Slug, a transcription factor of epithelial-mesenchymal transitions (EMT), is a molecular target that contributes to the biological specificity of c-kit signaling pathway. In this study, the expression of c-kit and Slug was evaluated in two ACC cell lines and 121 patients with ACC. The results of real-time RT-PCR and Western blot showed that ACC-2 and ACC-M cell lines expressed c-kit and Slug mRNA and protein. The immunohistochemical assay in patients demonstrated that positive expression of c-kit and Slug was observed in 108/121 (89.26%) and 87/121 (71.90%) of cases, respectively, and that c-kit and Slug expression was significantly associated with tumor site, TNM stage, histological pattern, perineural invasion, local regional recurrence and distant metastasis of patients with ACC (P