Suppressor factor from a T cell hybrid inhibits delayed-type hypersensitivity responses to azobenzenearsonate.
Suppressor factor from a T cell hybrid inhibits delayed-type hypersensitivity responses to azobenzenearsonate.
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来自 T 细胞杂交体的抑制因子可抑制对偶氮苯胂酸盐的迟发型超敏反应。
DOI：
10.1073/pnas.78.10.6441
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发表时间：
1981
影响因子：
11.1
通讯作者：
Benacerraf,B
中科院分区：
文献类型：
--
作者：
Whitaker,RB;Nepom,JT;Sy,MS;Takaoki,M;Gramm,CF;Fox,I;Germain,RN;Nelles,MJ;Greene,MI;Benacerraf,B
以聚乙二醇1540为诱导剂，将BW5147 AKR T淋巴瘤细胞与A/J小鼠脾细胞融合，诱导出ABA特异性抑制性T细胞。在最初播种的576个微孔中，132个显示出正在生长的细胞克隆，其中4个产生了ABA结合的上清因子。当在体内测试对ABA的迟发性超敏反应的抑制时，这些细胞系中的两个，A4和F12，被证明产生抑制上清因子。用适当的抗血清对F12细胞的荧光分析表明，该T细胞杂交体为Thy 1.2+，Lyt 1+，2-，表面免疫球蛋白阴性，这是传统ABA特异性抑制T细胞的表面标志表型。这种克隆的抑制细胞系F12产生一种稀释度高达1：100的培养上清液因子，并为遗传和免疫化学分析提供了材料。
By using polyethylene glycol 1540, BW5147 AKR T lymphoma cells were fused with splenocytes from A/J mice treated so as to induce suppressor T cells specific for azobenzenearsonate (ABA). Of 576 microwells originally seeded, 132 demonstrated growing cell clones, 4 of which produced an ABA-binding supernatant factor. When tested in vivo for suppression of delayed-type hypersensitivity to ABA, two of these cell lines, A4 and F12, were shown to produce suppressive supernatant factors. Fluorescence analysis of the F12 cells with appropriate antisera demonstrated this T cell hybrid to be Thy 1.2+, Lyt 1+,2-, and surface immunoglobulin negative, the surface marker phenotype of conventional ABA-specific suppressor T cells. This cloned suppressor cell line, F12, produces a culture supernatant factor that is suppressive at dilutions up to 1:100 and has provided material for genetic and immunochemical analysis.