Movements of epithelial cell sheets in vitro.
Movements of epithelial cell sheets in vitro.
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上皮细胞片的体外运动。
DOI：
10.1007/bf01935891
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发表时间：
1966
影响因子：
4
通讯作者：
J. Trinkaus
中科院分区：
文献类型：
--
作者：
R. B. Vaughan;J. Trinkaus
鸡胚上皮细胞，在体外培养的平面玻璃表面上，显示皱褶的膜活动，如在成纤维细胞。当相似的上皮细胞接触时，形成持久的粘连，这与膜活动的停止有关。上皮细胞片的移动与部分或全部边缘细胞对基质的粘附相关。这种粘附在没有膜活性的情况下不会发生。非边缘细胞通常表现为不粘附于基质;然而，偶尔，当与片层中相邻细胞的附着被破坏时，它们变得粘附。这种行为伴随着明显的膜活性。边缘细胞的移动和定向是细胞片层运动的重要步骤。
Chick embryo epithelial cells, cultured in vitro on a plane glass surface, show ruffled membrane activity like that seen in fibroblasts. When similar epithelial cells come into contact, long-lasting adhesions form, which are associated with the cessation of membrane activity. Movement of epithelial cell sheets is correlated with adhesion to the substratum of some or all of the marginal cells. Such adhesion does not occur in the absence of membrane activity. Non-marginal cells ordinarily appear to be non-adherent to the substratum; occasionally, however, they become adherent when attachments to neighbouring cells in a sheet are broken. This behaviour is accompanied by marked membrane activity. Mobilization and orientation of marginal cells are the vital steps in movements of coherent cell sheets.