Mechanoenzymatic reactions with whole cell transaminases: shaken, not stirred
Mechanoenzymatic reactions with whole cell transaminases: shaken, not stirred
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DOI：
10.1039/d2gc01006b
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发表时间：
2022-04-11
期刊：
影响因子：
9.8
通讯作者：
Hailes, Helen C.
中科院分区：
文献类型：
--
作者：
Carter, Eve M.;Ambrose-Dempster, Esther;Hailes, Helen C.
机械力化学反应是近年来出现的一种绿色合成方法，因为与传统的合成方法相比，机械力化学反应可以更快地进行，并且使用更少的溶剂。迄今为止，很少有机械酶促反应被描述。第一次，广泛用于醛和酮的胺化的转氨酶，已在机械酶促条件下用于生产胺，使用比常规生物催化反应显著更少的水介质。直接使用全细胞也是可能的，并且可以使用较短的反应时间以高效地提供具有高产率和立体选择性的胺。
Mechanochemical reactions have emerged in recent years as a green synthetic method because reactions can be performed more rapidly and using less solvent than traditional synthetic approaches. To date, very few mechanoenzymatic reactions have been described. For the first time, transaminases, which are widely used for the amination of aldehydes and ketones, have been used here under mechanoenzymatic conditions to produce amines using significantly less aqueous medium than conventional biocatalytic reactions. The direct use of whole cells was also possible and shorter reaction times could be used to provide amines efficiently with high yields and stereoselectivities.