In vitro susceptibility of contagious ovine digital dermatitis associated Treponema spp. isolates to antimicrobial agents in the UK.
In vitro susceptibility of contagious ovine digital dermatitis associated Treponema spp. isolates to antimicrobial agents in the UK.
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DOI：
10.1111/vde.12269
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发表时间：
2015-12
影响因子：
1.4
通讯作者：
Evans NJ
中科院分区：
文献类型：
--
作者：
Angell JW;Clegg SR;Sullivan LE;Duncan JS;Grove-White DH;Carter SD;Evans NJ
羊传染性趾皮炎（CODD）是英国和爱尔兰羊传染性跛行的重要原因，对受影响个体的福利有严重影响。三种密螺旋体群：中等密螺旋体/类文森特密螺旋体、类噬菌体密螺旋体和足密螺旋体螺旋体与临床CODD病变相关，被认为是疾病的必要原因。关于从CODD病变培养的密螺旋体的抗菌药物敏感性的数据很少。本研究的目的是在体外测定羊业中使用的抗菌剂对三种CODD相关密螺旋体群（中等密螺旋体/类文森特密螺旋体、类噬菌体密螺旋体和足密螺旋体）分离株的最小抑制浓度/最小杀菌浓度（MIC/MBC）。20个密螺旋体分离株;来自19只患有临床CODD病变的绵羊。采用微量稀释法测定了10种抗菌剂对20株密螺旋体分离株（5株培养基/类文森特密螺旋体、10株噬菌体样密螺旋体和5株足密螺旋体）的体外MIC/MBC。检测的抗菌剂为青霉素G、阿莫西林、土霉素、替米考星、林可霉素、大观霉素、泰乐菌素、泰地罗新、土拉霉素和加米霉素。检测的密螺旋体分离株对所有10种检测的抗菌剂均显示出较低的MIC和MBC。对加米霉素和替地罗新最敏感（MIC 90：0.0469 mg/L），对林可霉素、大观霉素和土霉素最不敏感（MIC 90：48 mg/L、24 mg/L和3 mg/L）。这些数据与从牛指皮炎病变培养的密螺旋体的体外抗菌药物敏感性数据相当。根据当地许可，青霉素和替米考星似乎是未来体内研究的最佳候选药物。 背景-传染性绵羊指皮炎（CDD）是英国和爱尔兰绵羊传染性跛行的重要原因，对受影响个体的福利有严重影响。三种密螺旋体群：中等密螺旋体/类文森特密螺旋体、类噬菌体密螺旋体和足密螺旋体螺旋体与临床CODD病变相关，被认为是疾病的必要原因。关于从CODD病变培养的密螺旋体的抗菌药物敏感性的数据很少。目的-本研究的目的是确定羊业中使用的抗菌剂对三种CODD相关密螺旋体群T. medium/T. vincentii‐like、T. phagedenis‐like和T. pedis.Conclusions分离株的体外MIC/MBC。这些数据与从牛指皮炎病变中培养的密螺旋体的体外抗菌药物敏感性数据相当。根据当地许可，青霉素和替米考星似乎是未来体内研究的最佳候选药物。
Contagious ovine digital dermatitis (CODD) is an important cause of infectious lameness in sheep in the UK and Ireland and has a severe impact on the welfare of affected individuals. The three treponemal phylogroups Treponema medium/Treponema vincentii‐like, Treponema phagedenis‐like and Treponema pedis spirochaetes have been associated with clinical CODD lesions and are considered to be a necessary cause of disease. There are scant data on the antimicrobial susceptibility of the treponemes cultured from CODD lesions. The aim of this study was to determine in vitro the miniumum inhibitory concentration/ minimum bactericidal concentration (MIC/MBC) of antimicrobials used in the sheep industry for isolates of the three CODD associated treponeme phylogroups T. medium/T. vincentii‐like, T. phagedenis‐like and T. pedis. Twenty treponeme isolates; from 19 sheep with clinical CODD lesions. A microdilution method was used to determine in vitro the MIC/MBC of 10 antimicrobial agents for 20 treponeme isolates (five T. medium/T. vincentii‐like, 10 T. phagedenis‐like and five T. pedis). The antimicrobials tested were penicillin G, amoxicillin, oxytetracycline, tilmicosin, lincomycin, spectinomycin, tylosin, tildipirosin, tulathromycin and gamithromycin. The treponeme isolates tested showed low MICs and MBCs to all 10 antimicrobials tested. They were most susceptible to gamithromycin and tildipirosin (MIC90: 0.0469 mg/L), and were least susceptible to lincomycin, spectinomycin and oxytetracycline (MIC90: 48 mg/L, 24 mg/L and 3 mg/L, respectively). These data are comparable to in vitro antimicrobial susceptibility data for treponemes cultured from bovine digital dermatitis lesions. Dependent on local licensing, penicillin and tilmicosin appear to be the best candidates for future in vivo studies. Background – Contagious ovine digital dermatitis (CODD) is an important cause of infectious lameness in sheep in the UK and Ireland and has a severe impact on the welfare of affected individuals. The three treponemal phylogroups Treponema medium/Treponema vincentii‐like, Treponema phagedenis‐like and Treponema pedis spirochaetes have been associated with clinical CODD lesions and are considered to be a necessary cause of disease. There is scant data on the antimicrobial susceptibility of the treponemes cultured from CODD lesions. Objective – The aim of this study was to determine in vitro the MIC/MBC of antimicrobials used in the sheep industry for isolates of the three CODD‐associated treponeme phylogroups T. medium/T. vincentii‐like, T. phagedenis‐like and T. pedis.Conclusions – These data are comparable to in vitro antimicrobial susceptibility data for treponemes cultured from bovine digital dermatitis lesions. Dependent on local licensing, penicillin and tilmicosin appear the best candidates for future in vivo studies.