Characterization of the transforming gene of Fujinami sarcoma virus.
Characterization of the transforming gene of Fujinami sarcoma virus.
复制标题
藤波肉瘤病毒转化基因的表征。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
H. Hanafusa
中科院分区：
文献类型：
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作者：
T. Hanafusa;L. Wang;S. Anderson;R. Karess;W. S. Hayward;H. Hanafusa
所有禽肉瘤病毒中存在的src基因并不存在于Fujinami肉瘤病毒的基因组中，Fujinami肉瘤病毒是一种在鸡身上产生肉瘤的有效病毒。Fujinami病毒是有缺陷的，需要辅助病毒进行复制。来自辅助病毒和转化病毒混合物的RNA由两个组分组成，35S和28S。每个RNA组分的寡核苷酸指纹图谱显示，35S组分与辅助病毒的RNA相同。因此，Fujinami病毒的基因组必须是28S RNA，其分子量大约相当于1.7×10(6)或5300个核苷酸。Fujinami病毒RNA在3‘端和5’端都与辅助病毒RNA共享几个寡核苷酸，但在基因组中间包含至少3000个核苷酸的独特序列。Fujinami病毒RNA不包含Rous肉瘤病毒基因组中src特异的寡核苷酸，也不与src序列互补的DNA杂交。在Fujinami病毒转化的细胞中检测不到Rous肉瘤病毒60,000道尔顿src蛋白。相反，这些转化的细胞含有一种14万道尔顿的蛋白质，可以被抗病毒蛋白的抗血清沉淀，这很可能是这种病毒的转化蛋白。
The src gene present in all avian sarcoma viruses is not present in the genome of Fujinami sarcoma virus, a potent sarcoma-inducing virus in chickens. Fujinami virus is defective and requires helper virus for replication. RNA from a mixture of helper and transforming viruses consists of two components, 35S and 28S. Oligonucleotide fingerprinting of each RNA component revealed that the 35S component was identical to the RNA of the helper virus. Thus, the genome of Fujinami virus must be the 28S RNA, which corresponds approximately to a molecular weight of 1.7 x 10(6) or 5300 nucleotides. Fujinami viral RNA shares several oligonucleotides with helper viral RNA at both 3' and 5' ends but contains a unique sequence of at least 3000 nucleotides in the middle of the genome. Fujinami viral RNA contains no src-specific oligonucleotides of the Rous sarcoma virus genome and did not hybridize with DNA complementary to the src sequences. The 60,000-dalton src protein of Rous sarcoma virus was undetectable in Fujinami virus-transformed cells. Instead, these transformed cells contain a protein of 140,000 daltons precipitable by antisera against virion proteins, which is likely to be the transforming protein of this virus.