The entire nucleotide sequence of two hepatitis G virus isolates belonging to a novel genotype: isolation in Myanmar and Vietnam
The entire nucleotide sequence of two hepatitis G virus isolates belonging to a novel genotype: isolation in Myanmar and Vietnam
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DOI：
10.1099/0022-1317-81-1-189
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发表时间：
2000-01-01
影响因子：
3.8
通讯作者：
Abe, K
中科院分区：
文献类型：
--
作者：
Naito, H;Hayashi, S;Abe, K
最近在东南亚国家受试者的血清中发现了一种新型庚型肝炎病毒（HGV）基因型。这些分离株分别从缅甸人（命名为HGV-MY 14）和越南人（命名为HGV-VT 48）受试者的血清中回收。为了更详细地表征病毒基因组，克隆了属于新基因型的两种不同HGV分离株的全长核苷酸序列。两株HGV均由9228个核苷酸组成，开放阅读框为8529个核苷酸，编码2843个氨基酸残基。这些分离株与先前报道的HGV/GBV-C分离株1至3型的核苷酸序列和氨基酸序列分别相差13-15%和4-6%。这两个分离株的推定的核心区域是不清楚地识别，因为它只由16个氨基酸残基。基于全长基因组序列和5 '-UTR序列的系统发育分析，HGV-MY 14和HGV-VT 48分离物可以被分类为新的基因型，命名为4型。
A novel genotype of hepatitis G virus (HGV) was recently identified in sera of subjects from countries in South-East Asia. These isolates were recovered from serum of Myanmarese (designated HGV-MY14) and Vietnamese (designated HGV-VT48) subjects, respectively. To characterize the viral genome in more detail, the full-length nucleotide sequence of the two different HGV isolates belonging to the novel genotype was cloned. Both HGV isolates were composed of 9228 nt and had a single open reading frame spanning 8529 nt and encoding 2843 aa residues. The isolates differed from previously reported HGV/GBV-C isolates types 1 to 3 by 13-15% (nucleotide sequence) and 4-6% (amino acid sequence). The putative core region of both isolates was not clearly identifiable as it consisted of only 16 aa residues. Based on phylogenetic analysis of full-length genome sequences and 5'-UTR sequences, HGV-MY14 and HGV-VT48 isolates can be classified as a novel genotype, designated type 4.